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La determinación del sexo dependiente de la temperatura está mediada por la represión pSTAT3 de Kdm6b

Ceri Weber¹, Yingjie Zhou², Jong Gwan Lee¹

¹Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.

Science (New York, N.Y.)

|April 18, 2020

Resumen

La determinación del sexo dependiente de la temperatura en las tortugas está regulada por Kdm6b. Las temperaturas más cálidas activan STAT3, que reprime Kdm6b, bloqueando el desarrollo masculino y promoviendo el desarrollo femenino.

Área de la Ciencia:

Biología del desarrollo
La epigenética
Endocrinología de reptiles

Sus antecedentes:

La determinación del sexo dependiente de la temperatura (TSD) es un factor clave en las estrategias reproductivas de los reptiles.
El regulador epigenético Kdm6b es crucial para activar la vía de desarrollo masculina en las especies de TSD.
Investigaciones anteriores indicaron una expresión elevada de Kdm6b a temperaturas de producción de machos.

Objetivo del estudio:

Establecer un vínculo causal directo entre la temperatura de incubación y la regulación transcripcional de Kdm6b.
Para aclarar los mecanismos moleculares por los cuales la temperatura influye en las vías de determinación del sexo.
Identificar las moléculas de señalización clave involucradas en la detección de la temperatura y la regulación genética.

Principales métodos:

Investigó el papel del transductor de señal y activador de la transcripción 3 (STAT3) en la regulación de Kdm6b.
Se utilizaron técnicas moleculares para evaluar la fosforilación de STAT3 y la unión al ADN en el locus Kdm6b.
Flujo cuantificado de iones de calcio (Ca2+) bajo diferentes condiciones de temperatura.

Principales resultados:

La fosforilación de STAT3 se observó a temperaturas más cálidas, produciendo hembras.
Se encontró que STAT3 fosforilado se une al locus del gen Kdm6b, reprimiendo su transcripción.
El influjo elevado de Ca2+ a las temperaturas de producción femenina media la activación de STAT3, bloqueando así la vía masculina.

Conclusiones:

Un mecanismo molecular directo enlaza la temperatura ambiente con la regulación de Kdm6b a través de STAT3.
STAT3 actúa como un represor sensible a la temperatura de la vía masculina al inhibir Kdm6b.
La señalización de calcio juega un papel crítico en la mediación de la activación de STAT3 inducida por la temperatura durante la embriogénesis de la tortuga.

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