Video Experimental Relacionado

Updated: Dec 22, 2025

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

Published on: September 1, 2015

Los interferones de tipo I inducidos por la microbiota instruyen un estado basal equilibrado de las células

Laura Schaupp¹, Sabine Muth², Leif Rogell³

¹Laboratory of Innate Immunity, Department of Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Hindenburgdamm 30, 12203 Berlin, Germany; Berlin Institute of Health (BIH), Anna-Louisa-Karsch Strasse 2, 10178 Berlin, Germany; Mucosal and Developmental Immunology, Deutsches Rheuma-Forschungszentrum, Charitéplatz 1, 10117 Berlin, Germany; Institute for Molecular Biology (IMB), Ackermannweg 4, 55128 Mainz, Germany.

|May 8, 2020

Resumen

La microbiota intestinal programa las células inmunes para la defensa de los patógenos. Este entrenamiento inmune, sin embargo, puede conducir a la activación de células T contra los propios tejidos del cuerpo, revelando una compensación evolutiva.

Área de la Ciencia:

Inmunología
Microbiología
Interacciones huésped-micróbio

Sus antecedentes:

Las señales ambientales, incluidas las de la microbiota, influyen en la fisiología del huésped y la preparación inmune.
Las células dendríticas convencionales (cDC) requieren señales de microbiota para la programación adecuada para iniciar la inmunidad adaptativa.
Los mecanismos moleculares de la programación de células inmunes guiadas por la microbiota siguen siendo en gran medida desconocidos.

Objetivo del estudio:

Investigar cómo la microbiota autóctona controla la función de las células inmunes, específicamente la producción de interferón de tipo I por DC plasmocitóides.
Esclarecer los fundamentos moleculares de la programación celular inmune inducida por la microbiota y su impacto en la defensa y la tolerancia del huésped.

Principales métodos:

Análisis del reguloma transcripcional y epigenético en todo el genoma.
Comparación de las CDC de ratones libres de gérmenes y con deficiencia de receptor de interferón alfa/beta (IFNAR).

Principales resultados:

La microbiota autóctona regula la producción de interferón constitutivo de tipo I (IFN-I) por las células plasmocitóides.
La señalización IFNAR tónica establece un estado epigenómico y metabólico específico en los CDC, preparándolos para los encuentros con patógenos.
Esta instrucción inmune viene con una compensación: las CDC instruidas pueden promover respuestas de células T contra autoantígenos, lo que potencialmente compromete la tolerancia periférica.

Conclusiones:

La microbiota huésped juega un papel crítico en la educación de las células inmunes, equilibrando la defensa del patógeno con la auto-tolerancia.
La programación inmune impulsada por la microbiota a través de la señalización IFNAR crea un estado de alta preparación inmune, pero conlleva el riesgo de autoinmunidad.
Estos hallazgos ofrecen información sobre los compromisos evolutivos inherentes a la adaptación de los vertebrados a sus simbiontes microbianos.

Palabras clave:

La microbiota las células dendríticas convencionales Tolerancia periférica Las células dendríticas plasmocitóides Interferones de tipo I

Más Videos Relacionados

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Published on: May 21, 2012

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Induction of Intestinal Inflammation by Adoptive Transfer of CBir1 TCR Transgenic CD4+ T Cells to Immunodeficient Mice

Published on: December 16, 2021

Videos de Conceptos Relacionados

Immune Response Against Viral Pathogens

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Cell-mediated Immune Responses

Cell-mediated Immune Responses

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

Cells of the Innate Immune Response

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...