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Mapeo personalizado del metabolismo de las drogas por el microbioma intestinal humano
Bahar Javdan1, Jaime G Lopez2, Pranatchareeya Chankhamjon1
1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Cell
|June 12, 2020
Resumen
El microbioma intestinal humano puede metabolizar las drogas, pero esto se entiende mal en comunidades complejas. Desarrollamos un marco novedoso para mapear el metabolismo de fármacos por los microbios intestinales, revelando interacciones específicas individuales para el desarrollo de fármacos.
Área de la Ciencia:
- Microbiología
- Farmacología
- Biología de sistemas
Sus antecedentes:
- El microbioma intestinal humano contiene numerosas bacterias con funciones metabólicas.
- Si bien las especies bacterianas individuales pueden metabolizar los medicamentos, el metabolismo a nivel comunitario está poco estudiado.
Objetivo del estudio:
- Desarrollar un marco cuantitativo para evaluar el metabolismo de los medicamentos por el microbioma intestinal humano.
- Identificar las interacciones entre fármacos y microbiomas y los genes responsables dentro de las comunidades microbianas.
Principales métodos:
- Se ha desarrollado una pantalla de metabolismo derivado del microbioma (MDM), un marco cuantitativo.
- Se utilizó un cultivo por lotes para las comunidades microbianas intestinales específicas del sujeto.
- Se utiliza el cribado ex vivo del metabolismo de los fármacos y la metagenómica funcional.
Principales resultados:
- Se han identificado nuevas interacciones fármaco-microbioma.
- Se ha demostrado que estas interacciones varían significativamente entre individuos.
- Descubrimiento de genes codificados por el microbioma responsables de eventos metabólicos específicos de medicamentos.
Conclusiones:
- El microbioma intestinal juega un papel importante en el metabolismo de fármacos de pequeñas moléculas.
- Las interacciones entre fármacos y microbiomas son personalizadas y dependen del contexto.
- Este marco puede promover el desarrollo de fármacos y la medicina personalizada.
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