Video Experimental Relacionado

Updated: Dec 18, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Regulación transcripcional de la señalización de las estrigolactonas en Arabidopsis

Lei Wang^1,2, Bing Wang³, Hong Yu¹

¹State Key Laboratory of Plant Genomics, and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.

|June 13, 2020

Resumen

Las estrigolactonas regulan el desarrollo de las plantas mediante el control de la transcripción génica. La proteína SMXL6 actúa como un factor de transcripción, reprimiendo los genes para mantener el equilibrio de la señalización hormonal.

Área de la Ciencia:

Biología vegetal
Biología molecular
La señalización hormonal

Sus antecedentes:

Las estrigolactonas son hormonas vegetales cruciales para el desarrollo y las interacciones vegetales.
Las proteínas clave como DWARF14 (D14) y MORE AXILLARY GROWTH 2 (MAX2) están involucradas en la señalización de la estrigolactona.
La regulación transcripcional aguas abajo por las estrigolactonas sigue siendo en gran medida no caracterizada.

Objetivo del estudio:

Para identificar los genes sensibles a la estrigolactona en la Arabidopsis thaliana.
Aclarar los mecanismos de transcripción subyacentes al desarrollo de las plantas mediado por la estrigolactona.
Investigar el papel de las proteínas SMXL en la señalización de la estrigolactona.

Principales métodos:

Utilizó una estrigolactona sintética para identificar genes receptivos a través del análisis transcriptómico.
Investigó la regulación genética de la ramificación del brote, la forma de la hoja y la acumulación de antocianina.
Se realizaron ensayos de inmunoprecipitación de cromatina y de unión al promotor para estudiar la función de SMXL6.

Principales resultados:

Se identificaron 401 genes sensibles a las estrigolactonas en Arabidopsis.
Se ha demostrado la regulación de la estrigolactona de los genes BRANCHED 1, TCP DOMAIN PROTEIN 1 y PRODUCTION OF ANTHOCYANIN PIGMENT 1.
Se muestra que SMXL6 reprime directamente la transcripción de SMXL6, SMXL7 y SMXL8, actuando como un factor de transcripción autorregulado.

Conclusiones:

Las estrigolactonas activan principalmente la transcripción descendente para controlar el desarrollo de las plantas.
SMXL6 funciona como un factor de transcripción de unión al ADN que autorregula la señalización de las estrigolactonas.
Reveló un nuevo mecanismo de regulación de la señalización hormonal a través de la represión transcripcional directa en las plantas.

Más Videos Relacionados

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Published on: August 25, 2018

Videos de Conceptos Relacionados

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Transcription

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

Biological Clocks and Seasonal Responses

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.