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Un IgSF humano Interactoma de la superficie celular revela una red compleja de interacciones proteína-proteína
Woj M Wojtowicz1, Jost Vielmetter2, Ricardo A Fernandes1
1Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Cell
|August 22, 2020
Resumen
Los investigadores mapearon más de 380 nuevas interacciones proteína-proteína de la superficie celular (PPI), revelando una compleja red de comunicación. Este descubrimiento mejora la comprensión de las funciones del sistema inmunológico y nervioso e identifica posibles objetivos terapéuticos.
Área de la Ciencia:
- Biología celular
- Inmunología
- La neurociencia
- Proteomía
Sus antecedentes:
- Las interacciones proteína-proteína de la superficie celular (IPP) son cruciales para la comunicación, el reconocimiento y las respuestas entre células.
- Comprender estas interacciones es vital para descifrar los procesos biológicos y los mecanismos de la enfermedad.
Objetivo del estudio:
- Examinar sistemáticamente las nuevas interacciones entre la superficie celular humana y las proteínas secretadas (IPP).
- Identificar nuevos socios de interacción tanto para receptores conocidos como para receptores huérfanos.
- Para construir un mapa interactivo completo de las proteínas de la superficie celular.
Principales métodos:
- Se utilizó una plataforma de detección automatizada basada en ELISA de alto rendimiento.
- Se realizó una exploración del interactoma de 564 proteínas de la superficie celular humana y secretadas, predominantemente proteínas de la superfamilia de las inmunoglobulinas (IgSF).
- Para la validación se emplearon análisis de homología filogenética, resonancia plasmónica superficial y ensayos de unión celular.
Principales resultados:
- El análisis identificó aproximadamente 380 IPP no notificados previamente entre las 318.096 combinaciones evaluadas.
- Se encontraron nuevas interacciones para los receptores con ligandos conocidos y para los receptores " huérfanos ".
- Los IBP identificados implican proteínas en diversas funciones, incluido el desarrollo del sistema inmunológico y nervioso, el metabolismo y la reproducción.
Conclusiones:
- Se ha descubierto una vasta e intrincada red de IPP de la superficie celular.
- Estas nuevas interacciones proporcionan un recurso valioso para investigar las funciones biológicas.
- Los IPP identificados representan objetivos potenciales para el desarrollo de fármacos terapéuticos.
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