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El canal permeable al calcio OSCA1.3 regula la inmunidad estomatal de las plantas

Kathrin Thor¹, Shushu Jiang^1,2, Erwan Michard³

¹The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.

|August 27, 2020

Resumen

La señalización inmune de las plantas desencadena el cierre estomático a través del canal de calcio (Ca2+) OSCA1.3. Este canal

Área de la Ciencia:

Biología vegetal
Patología molecular de las plantas
Fisiología de los canales iónicos

Sus antecedentes:

El cierre estomático en las plantas es una respuesta clave a las tensiones bióticas y abióticas.
El influjo de iones de calcio (Ca2+) es crucial para esta respuesta, pero se desconocen los canales específicos involucrados.
Comprender estos canales es vital para la inmunidad de las plantas y la respuesta al estrés.

Objetivo del estudio:

Identificar el canal de calcio responsable del cierre estomático durante la señalización inmune de la planta.
Elucidar el mecanismo de activación del canal por patrones moleculares asociados al patógeno (PAMP).

Principales métodos:

Utilizó la Arabidopsis thaliana como organismo modelo.
Se utilizaron ensayos bioquímicos y fosfoproteómica cuantitativa para identificar las interacciones de las proteínas y los sitios de fosforilación.
Se llevaron a cabo experimentos genéticos y electrofisiológicos para confirmar la función y regulación del canal.

Principales resultados:

Se identificó a OSCA1.3 como un canal permeable a Ca2+ que controla el cierre estomático durante la señalización inmune.
Se demostró que la quinasa BIK1 fosforila OSCA1.3 tras la percepción de PAMP, mejorando su actividad.
Se demostró que el cierre estomático mediado por OSCA1.3 es específico para la señalización inmune y no está involucrado en las respuestas del ácido abscísico.

Conclusiones:

OSCA1.3 es un canal crítico de Ca2+ para las respuestas inmunes de las plantas que conducen al cierre estomático.
La fosforilación mediada por BIK1 es un mecanismo regulador clave para la activación de OSCA1.3.
Este estudio revela especificidad en las vías de señalización de Ca2+ para diferentes respuestas de estrés de las plantas.

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