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brlA es necesario y suficiente para dirigir el desarrollo de conidióforos en Aspergillus nidulans
T H Adams1, M T Boylan, W E Timberlake
1Department of Genetics, University of Georgia, Athens 30602.
Cell
|July 29, 1988
Resumen
El gen brlA regula el desarrollo de Aspergillus nidulans, cambiando las células del crecimiento a la formación de esporas. Su expresión en células vegetativas desencadena la esporulación mediante la activación de genes específicos.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
- La micología de la micología.
Sus antecedentes:
- El gen brlA en Aspergillus nidulans controla la transición del crecimiento vegetativo al desarrollo de conidióforos.
- brlA codifica una proteína con motivos de unión al ADN similares a los de Xenopus TFIIIA.
Objetivo del estudio:
- Investigar el papel del gen brlA en la mediación de las transiciones del desarrollo en A. nidulans.
- Comprender la función de la proteína BRLA en la regulación de la expresión génica durante la esporulación.
Principales métodos:
- Análisis de los patrones de expresión génica.
- Observación de la morfología celular y los cambios en el desarrollo.
- Manipulación genética de la expresión de brlA.
Principales resultados:
- La expresión mal programada de brlA en células vegetativas indujo la activación transcripcional de genes de desarrollo.
- La expresión brlA condujo al cese del crecimiento hifático y al inicio de estructuras similares a los conidióforos.
- Se produjeron conidiosporas viables tras la expresión de brlA en células vegetativas.
Conclusiones:
- Se propone que la proteína BRLA sea una proteína de unión de ácido nucleico.
- La expresión de brlA en células vegetativas es suficiente para inducir la esporulación.
- BRLA regula la expresión de genes específicos de la conidiación, controlando el interruptor de desarrollo.
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