Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.6K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.6K
Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

3.1K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
3.1K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.8K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.8K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

8.6K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.6K
Membrane Domains01:18

Membrane Domains

6.8K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
6.8K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.3K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.3K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Mechanistic Insights into Pulmonary Surfactant Inactivation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Directional motion of a self-steering active intruder in a dense crowd of cognitive active agents.

Scientific reports·2026
Same author

Dynamic bidirectional coupling of membrane morphology and rod organization in flexible vesicles.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Do crowded phospholipid monolayers remain fluid?

Journal of the Royal Society, Interface·2026
Same author

Author Correction: Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis.

Nature plants·2026
Same author

Membrane remodelling mediates lipopeptide-induced immunity in Arabidopsis.

Nature plants·2026

Video Experimental Relacionado

Updated: Dec 7, 2025

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.5K

Las partículas activas inducen grandes deformaciones en las vesículas lipídicas gigantes

Hanumantha Rao Vutukuri1, Masoud Hoore2, Clara Abaurrea-Velasco2

  • 1Soft Materials, Department of Materials, ETH Zürich, Zürich, Switzerland. h.r.vutukuri@mat.ethz.ch.

Nature
|October 1, 2020
PubMed
Resumen

Las partículas autopropulsadas dentro de las vesículas unilamellares gigantes crean formas complejas y no equilibradas y fluctuaciones activas de la membrana. Esta investigación modela la dinámica de las membranas celulares y podría informar el diseño de células artificiales y robots blandos.

Más Videos Relacionados

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
05:43

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Published on: January 24, 2017

14.9K
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
09:29

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Published on: January 19, 2020

8.9K

Videos de Experimentos Relacionados

Last Updated: Dec 7, 2025

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.5K
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
05:43

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Published on: January 24, 2017

14.9K
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
09:29

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Published on: January 19, 2020

8.9K

Área de la Ciencia:

  • La biofísica
  • Física de la materia blanda
  • Mecánica celular

Sus antecedentes:

  • Las células biológicas esculpen activamente membranas internas para la detección e interacción con el medio ambiente.
  • Las bacterias patógenas utilizan fuerzas internas para deformar las membranas celulares del huésped para la invasión.
  • Las vesículas unilamellares gigantes sirven como modelos mínimos para las membranas celulares, pero crear fuerzas activas internas sigue siendo un desafío.

Objetivo del estudio:

  • Para investigar cómo las partículas autopropulsadas dentro de las vesículas unilamellares gigantes inducen deformaciones de la membrana y cambios de forma.
  • Explorar la relación entre las fuerzas activas internas y las morfologías de las vesículas emergentes.
  • Desarrollar un sistema de modelo mínimo capaz de esculpir membranas dinámicas.

Principales métodos:

  • Observación experimental de la respuesta de la membrana a los nadadores Janus autoforéticos utilizando microscopía confocal.
  • Simulaciones dinámicas de Langevin de partículas brownianas activas dentro de capas de membrana (superficies trianguladas dinámicamente).
  • Cuantificación de los cambios dinámicos de la membrana y las transformaciones de forma.

Principales resultados:

  • Las partículas autopropulsadas inducen diversas formas de no equilibrio y fluctuaciones activas de la membrana.
  • Las concentraciones de partículas bajas a moderadas conducen a protuberancias parecidas a tiras y estructuras dendríticas.
  • Las altas concentraciones de partículas dan lugar a formas de vesículas globalmente deformadas.
  • Se genera un diagrama de estado que predice los resultados de la forma en función de las condiciones de fuerza interna.

Conclusiones:

  • Las fuerzas activas internas de las partículas cerradas pueden provocar deformaciones de la membrana significativas y controlables.
  • El estudio proporciona un marco para la comprensión de la dinámica de la membrana activa en sistemas mínimos.
  • Los hallazgos pueden avanzar en el diseño de células sintéticas y robótica blanda a microescala.