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La proteína MAT alfa 1, un activador de la transcripción de la levadura, se une sinérgicamente con una segunda
Cell
|August 28, 1987
Resumen
La proteína alfa 1 de la levadura se une a regiones genéticas específicas, lo que requiere otra proteína llamada PRTF. Esta interacción crea la expresión génica alfa-específica, con PRTF también involucrado en la transcripción génica a-específica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética de la levadura Genética de la levadura
- Reglamento genético Reglamento genético.
Sus antecedentes:
- El locus del tipo de apareamiento de la levadura controla el tipo de célula y la reproducción sexual.
- Los genes alfa-específicos y a-específicos se regulan de manera diferente según el tipo de célula.
Objetivo del estudio:
- Investigar el mecanismo molecular por el cual la proteína alfa 1 confiere especificidad alfa en la expresión génica de la levadura.
- Para identificar las interacciones de proteínas involucradas en la regulación de los genes alfa-específicos y a-específicos.
Principales métodos:
- Ensayos de desplazamiento de movilidad por electroforesis (EMSA)
- Ensayos de huellas de ADNasa I.
- Experimentos vinculantes y de competencia con oligonucleótidos sintéticos.
Principales resultados:
- La proteína alfa 1 se une a secuencias homólogas en las regiones de control de genes alfa específicos.
- La unión requiere tanto alfa 1 como una segunda proteína, PRTF (Factor de Transcripción de Respuesta Feromónica).
- PRTF se une a una parte de la homología de la secuencia, mientras que alfa 1 se une al resto, lo que sugiere un mecanismo de unión cooperativo.
Conclusiones:
- Alpha 1 crea un sitio de unión para PRTF, lo que confiere especificidad alfa a la expresión génica.
- PRTF también está implicado en la transcripción de genes específicos.
- La proteína alfa 2 puede regular la a-especificidad mediante el bloqueo de los sitios de unión PRTF.
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