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Videos de Conceptos Relacionados

Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...

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Updated: May 7, 2026

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
11:31

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)

Published on: March 15, 2013

El lacrepresor es una proteína transitorio de activación génica.

S B Straney1, D M Crothers

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511.

Cell
|December 4, 1987
PubMed
Resumen

El lacrepresor mejora significativamente la unión inicial de la ARN polimerasa con el ADN promotor lac UV5 de E. coli. Esta estabilización vinculante, contrariamente a creencias anteriores, facilita una rápida respuesta del sistema a la liberación de la represión.

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Last Updated: May 7, 2026

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
11:31

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Published on: March 15, 2013

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Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética La genética.
  • La bioquímica es la bioquímica.

Sus antecedentes:

  • El represor lac se entiende tradicionalmente para inhibir la transcripción génica.
  • Se debate su papel preciso en las etapas iniciales de la iniciación de la transcripción en el promotor de E. coli lac UV5.

Objetivo del estudio:

  • Para investigar el efecto del lacrepresor en la unión inicial de la ARN polimerasa al promotor lac UV5 de E. coli.
  • Para aclarar el mecanismo por el cual el lacrepresor influye en la iniciación de la transcripción.

Principales métodos:

  • La combinación de métodos experimentales.
  • Estudios cinéticos de la iniciación de la transcripción.
  • Análisis de la unión entre la ARN polimerasa y el ADN.

Principales resultados:

  • El represor lac aumenta la unión de la ARN polimerasa al promotor lac UV5 en más de 100 veces.
  • El represor bloquea el paso de isomerización en la iniciación de la transcripción.
  • La adición de IPTG permite la formación y transcripción de complejos abiertos, con el represor y el inductor aumentando la velocidad de la primera ronda de transcripción productiva a bajas concentraciones de polimerasa.

Conclusiones:

  • El represor lac juega un doble papel: estabilizar los complejos pre-transcripcionales y bloquear la iniciación.
  • Este doble papel sugiere un modelo regulatorio más amplio que la simple represión.
  • Los hallazgos desafían la visión convencional de la función del lacrepresor en la transcripción.