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Plegamiento de un péptido de unión al hierro intrínsecamente desordenado en respuesta a la sedimentación revelada por

Geula Davidov^1,2, Gili Abelya¹, Ran Zalk²

¹Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel.

Journal of the American Chemical Society

|November 9, 2020

Resumen

Las proteínas de biomineralización, a menudo intrínsecamente desordenadas, controlan la formación de minerales. Este estudio visualiza la proteína M6A interactuando con nanopartículas de óxido de hierro, revelando cómo las proteínas desordenadas adquieren estructura al contacto con minerales.

Área de la Ciencia:

La bioquímica
Biología estructural
Ciencias de los materiales

Sus antecedentes:

La biomineralización se basa en proteínas especializadas para regular la deposición de minerales.
Las proteínas intrínsecamente desordenadas (IDP) juegan un papel clave, pero sus estructuras de alta resolución durante la interacción mineral son poco conocidas.

Objetivo del estudio:

Visualizar la interacción de alta resolución entre una proteína intrínsecamente desordenada (M6A) y una partícula de óxido de hierro.
Para entender cómo los IDP controlan la microestructura mineral durante la biomineralización.

Principales métodos:

Utilizó un nanorreactor de ferritina para la interacción proteína-mineral controlada.
Se empleó microscopía crioelectrónica (crio-EM) para visualizar el complejo.
Desarrolló un nuevo método para determinar la forma de la partícula basada en las diferencias de sensibilidad de la dosis de electrones.

Principales resultados:

Visualizó la interacción entre M6A y las nanopartículas de óxido de hierro en alta resolución.
El plegamiento observado de M6A está correlacionado con la detección de partículas minerales.
Se demostró que la interacción C-terminal de M6A estabiliza una hélice N-terminal, mostrando una respuesta IDP al entorno.

Conclusiones:

Proporciona los primeros conocimientos estructurales de alta resolución sobre la biomineralización mediada por el IDP.
Revela un mecanismo en el que los PDI sufren cambios conformacionales al interactuar con minerales, lo que influye en la microestructura mineral.
Destaca el papel de los PDI en el control de la formación de partículas minerales nacientes.

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