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Respuesta al comentario sobre "Los orígenes antiguos de la activación alostérica en una Ser-Thr quinasa"

Christopher Wilson¹, Dorothee Kern²

¹Department of Biochemistry and Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02453, USA.

Science (New York, N.Y.)

|November 20, 2020

Resumen

Park y otros. cuestionar la regulación alostérica de Aurora por el TPX2. Anteriormente abordamos las preocupaciones sobre el muestreo de secuencias y los métodos de árbol genético. Nuestro análisis sugiere un antepasado no alostérico y pone de relieve los problemas con los árboles genéticos forzados por especies.

Área de la Ciencia:

Biología evolutiva
Biología molecular
La genómica

Sus antecedentes:

La regulación alostérica es crucial para la función de las proteínas.
Las auroras cinasas y las interacciones TPX2 son claves en la división celular.
Estudios previos proponían la regulación alostérica de Aurora por TPX2.

Objetivo del estudio:

Para abordar las críticas con respecto a la regulación alostérica de Aurora por TPX2.
Para reevaluar los métodos de reconstrucción de secuencias ancestrales.
Investigar el impacto de las metodologías del árbol genético en las conclusiones evolutivas.

Principales métodos:

Reconstrucción de la secuencia ancestral.
Análisis filogenético utilizando árboles de especies y árboles de genes.
Comparación de secuencias y análisis de divergencias evolutivas.

Principales resultados:

Anteriormente se resolvieron las preocupaciones sobre el muestreo de secuencias y los métodos de árbol genético.
La reconstrucción de la secuencia ancestral apoya una interacción no alostérica Aurora-TPX2.
Los árboles de genes forzados por especies introducen diferencias de secuencia significativas, lo que podría tergiversar la historia evolutiva.

Conclusiones:

La regulación alostérica de Aurora por TPX2 es poco probable.
Las elecciones metodológicas en el análisis filogenético pueden impactar significativamente las inferencias evolutivas.
Se requieren más investigaciones sobre los mecanismos precisos de regulación de las quinasas Aurora.

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