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Comentario sobre "Los orígenes antiguos de la activación alostérica en una Ser-Thr quinasa"

Yeonwoo Park¹, Jaeda E J Patton¹, Georg K A Hochberg²

¹Committee on Genetics, Genomics and Systems Biology, University of Chicago, Chicago, IL, USA.

Science (New York, N.Y.)

|November 20, 2020

Resumen

Las quinasas auroras desarrollaron la regulación alostérica a través de sustituciones de secuencias históricas. Sin embargo, este estudio revela que los hallazgos anteriores se basaron en árboles evolutivos defectuosos y datos limitados, lo que sugiere que la alostería se perdió, no se ganó, en algunos hongos.

Área de la Ciencia:

Biología evolutiva
La bioquímica
La genómica

Sus antecedentes:

Las quinasas auroras son reguladores cruciales de la división celular.
Investigaciones anteriores sugirieron que las quinasas Aurora desarrollaron regulación alosterica durante la evolución eucariota temprana.
La reconstrucción de secuencias ancestrales es un método para inferir secuencias de proteínas ancestrales.

Objetivo del estudio:

Para reevaluar la historia evolutiva de la regulación alostérica en las quinasas Aurora.
Identificar posibles inexactitudes en los métodos de reconstrucción de secuencias ancestrales anteriores.
Para determinar si la regulación alostérica fue ganada o perdida durante la evolución.

Principales métodos:

Análisis filogenético utilizando árboles evolutivos actualizados.
Genómica comparativa con muestreo de secuencias expandidas.
Reconstrucción de la secuencia ancestral.

Principales resultados:

La filogenia utilizada por Hadzipasic et al. fue hallado inverosímil.
El escaso muestreo de secuencias condujo a reconstrucciones ancestrales inexactas.
El nuevo análisis indicó que la regulación alostérica probablemente se perdió en un subgrupo de hongos, no se ganó en eucariotas.

Conclusiones:

La evolución de la regulación alostérica en las quinasas Aurora requiere una reevaluación.
Los métodos filogenéticos y de muestreo inexactos pueden conducir a inferencias evolutivas erróneas.
La regulación alostérica en las quinasas Aurora puede representar una pérdida en ciertos linajes fúngicos en lugar de una ganancia en los eucariotas tempranos.

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