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Abl y Canoe/Afadin median la mecanotransducción en las uniones tricelulares
Huapeng H Yu1, Jennifer A Zallen2
1Howard Hughes Medical Institute and Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA.
Science (New York, N.Y.)
|November 27, 2020
Resumen
Los científicos descubrieron una nueva vía en la que Abl tirosina quinasa y Canoe / Afadin estabilizan la adhesión celular bajo tensión en embriones de mosca de la fruta, revelando cómo las células responden a las fuerzas mecánicas durante el desarrollo de los tejidos.
Área de la Ciencia:
- Biología celular
- Biología del desarrollo
- La biofísica
Sus antecedentes:
- Los tejidos epiteliales reorganizan dinámicamente las células en respuesta a las fuerzas mecánicas.
- Las uniones de adherencias son clave para la transmisión de fuerzas entre las células.
- Los mecanismos in vivo de detección y respuesta de la fuerza celular siguen siendo incompletamente entendidos.
Objetivo del estudio:
- Identificar los mecanismos moleculares por los que las células perciben y responden a la tensión mecánica en las uniones tricelulares.
- Para aclarar el papel de la tirosina quinasa Abl y Canoe / Afadin en la mecanotransducción epitelial.
Principales métodos:
- Se ha investigado la mecanotransducción en embriones de *Drosophila*.
- Utilizó la manipulación genética para estudiar las funciones de Abl kinase y Canoe/Afadin.
- Se examinó la localización de proteínas y la dinámica de fosforilación en las uniones tricelulares.
- Se evaluó el impacto de la función alterada de Canoe en la adhesión celular y la remodelación epitelial.
Principales resultados:
- Identificó una vía que involucra a Abl tirosina quinasa y Canoe / Afadin que estabiliza la adhesión celular bajo tensión en las uniones tricelulares.
- Se demostró que el reclutamiento de Canoe a las uniones tricelulares se desencadena por la contractilidad de la actomiosina.
- Se ha demostrado que la fosforilación dependiente de Abl de Canoe es crucial para su mecanosensibilidad.
- Descubrió que la fosforilación alterada de Canoe desestabiliza la adhesión tricelular, mientras que la localización forzada detiene la reorganización celular.
Conclusiones:
- Descubrió un nuevo mecanismo de respuesta a la fuerza que regula la estabilidad de la adhesión tricelular durante la remodelación epitelial.
- La fosforilación dependiente de Abl de Canoe actúa como un sensor y transductor clave de la tensión mecánica.
- Esta vía es esencial para mantener la integridad epitelial y permitir reorganizaciones celulares dinámicas.
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