Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

14.1K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.1K
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

1.2K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
1.2K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

3.5K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
3.5K
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

1.8K
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
1.8K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

15.4K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
15.4K
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

16.6K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
16.6K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Addressing Biases in Analysis of Time of Infusion: NCI/SWOG Trial S1404 Among Participants With High-Risk Resectable Melanoma Who Received Adjuvant Anti-PD-1 Therapy.

JCO oncology practice·2026
Same author

Activation-dependent lentiviruses enable antigen-specific T cell expansion and transduction.

bioRxiv : the preprint server for biology·2026
Same author

Whole-protein screening and multi-modal profiling of antigen-specific CD4<sup>+</sup> T cells at single-cell resolution.

Nature communications·2026
Same author

MONETTE: A Randomized Phase II Study of Ceralasertib plus Durvalumab or Ceralasertib Monotherapy in Patients with Advanced Melanoma Resistant to PD-(L)1 Inhibition.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Cellular neighborhoods govern antitumor T-cell infiltration following anti-CTLA-4 in melanoma with primary resistance to anti-PD-1.

Cancer discovery·2026
Same author

Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer.

Nature communications·2026

Video Experimental Relacionado

Updated: Nov 28, 2025

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
09:08

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice

Published on: July 11, 2016

9.9K

Programas precisos de reconocimiento de células T diseñados mediante la vinculación transcripcional de múltiples

Jasper Z Williams1,2,3, Greg M Allen1,2,3,4, Devan Shah1,2,3

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.

Science (New York, N.Y.)
|November 27, 2020
PubMed
Resumen

Las células T diseñadas reconocen con precisión las células objetivo mediante la integración de múltiples señales de antígeno utilizando receptores sintéticos de Notch. Este sistema multirreceptor logra una focalización selectiva del tumor, demostrando capacidades avanzadas de reconocimiento celular.

Más Videos Relacionados

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
08:48

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

8.8K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

719

Videos de Experimentos Relacionados

Last Updated: Nov 28, 2025

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
09:08

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice

Published on: July 11, 2016

9.9K
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
08:48

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain

Published on: October 25, 2016

8.8K
Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
06:10

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

719

Área de la Ciencia:

  • Biología sintética
  • Ingeniería celular
  • Inmunología

Sus antecedentes:

  • El reconocimiento celular se basa en la integración de señales de múltiples receptores para la precisión.
  • Las interacciones moleculares individuales a menudo carecen de la precisión para tareas complejas de reconocimiento biológico.
  • La biología sintética ofrece herramientas para diseñar nuevas funciones celulares.

Objetivo del estudio:

  • Para diseñar circuitos de reconocimiento celular de múltiples receptores utilizando receptores sintéticos Notch.
  • Para permitir que las células T diseñadas integren múltiples eventos de reconocimiento de antígenos.
  • Para lograr un reconocimiento celular preciso y robusto con lógica sintonizable.

Principales métodos:

  • Diseñó una biblioteca de receptores sintéticos de Notch para crear interconexiones de transcripción.
  • Vías integradas de reconocimiento de antígenos extra e intracelulares.
  • Puertas lógicas positivas y negativas para la integración de antígenos.
  • He probado un circuito de tres antígenos y una puerta in vivo para detectar tumores.

Principales resultados:

  • Desarrolló circuitos sintéticos capaces de integrar hasta tres antígenos diferentes.
  • Robustez demostrada para la heterogeneidad celular.
  • Las células T diseñadas mostraron un reconocimiento preciso basado en señales de antígeno integradas.
  • Una puerta AND de tres antígenos limpió selectivamente tumores de tres antígenos in vivo, evitando tumores de dos antígenos.

Conclusiones:

  • Los circuitos basados en receptores sintéticos de Notch permiten un reconocimiento celular sofisticado mediado por múltiples receptores.
  • La cadena de eventos de reconocimiento molecular es una poderosa estrategia para diseñar el comportamiento celular.
  • Este enfoque mejora la especificidad y la precisión en las terapias celulares de ingeniería, particularmente para el tratamiento del cáncer.