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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

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Published on: March 16, 2011

La selección mejora la evolutividad de las proteínas al aumentar la robustez mutacional y la plegabilidad

Jia Zheng^1,2, Ning Guo³, Andreas Wagner^4,2,5

¹Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland.

Science (New York, N.Y.)

|December 4, 2020

Resumen

Una selección natural más fuerte mejora la evolución de una población, acelerando la evolución de nuevos rasgos. Esto ocurre al aumentar la robustez de la mutación y la plegabilidad de la proteína, allanando el camino para el éxito evolutivo.

Área de la Ciencia:

Biología evolutiva
Evolución molecular

Sus antecedentes:

La selección natural influye en la capacidad de evolución de una población, su capacidad de evolución adaptativa.
Los mecanismos que vinculan la fuerza de selección con la evolutividad siguen sin estar claros.

Objetivo del estudio:

Investigar cómo las diferentes fuerzas de selección afectan a la evolutividad.
Comprender la base molecular de la evolución mejorada bajo una selección fuerte.

Principales métodos:

Evolución dirigida de las poblaciones de proteínas fluorescentes amarillas.
Aplicación de diferentes regímenes de selección (fuerte y débil) para la fluorescencia amarilla.
Evolución posterior hacia un fenotipo de fluorescencia verde.

Principales resultados:

Las poblaciones bajo fuerte selección para la fluorescencia amarilla evolucionaron la fluorescencia verde más rápido.
La fuerte selección promovió mutaciones que mejoran la robustez y la plegabilidad de las proteínas.
La selección débil favoreció inicialmente la neofuncionalización, pero fue obstaculizada por mutaciones perjudiciales.

Conclusiones:

La selección natural puede mejorar significativamente la evolución.
El aumento de la robustez y la plegabilidad son mecanismos clave mediante los cuales la selección fuerte promueve la evolución adaptativa.
La fuerza de la selección es un factor crítico para determinar las trayectorias evolutivas y el éxito.

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