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G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
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Cómo los patrones de fosforilación de GPCR orquestan la señalización mediada por arrestina

Naomi R Latorraca1, Matthieu Masureel2, Scott A Hollingsworth3

  • 1Department of Computer Science, Stanford University, Stanford, CA 94305, USA; Institute for Computational and Mathematical Engineering, Stanford University, Stanford, CA 94305, USA; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA; Biophysics Program, Stanford University, Stanford, CA 94305, USA.

Cell
|December 9, 2020
PubMed
Resumen

Los patrones de fosforilación de las GPCR, no sólo el número de fosfatos, dictan los cambios de unión y conformación. Esto revela la base estructural para la detención

Palabras clave:
Simulación de la dinámica molecular de todos los átomosseñalización sesgadaSelectividad funcionalModificación después de la traducciónInteracciones proteína-proteínaReceptor de siete membranas

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Área de la Ciencia:

  • La bioquímica
  • Señalización celular
  • Biología estructural

Sus antecedentes:

  • La unión de Arrestin a los receptores acoplados a proteínas G fosforiladas (GPCR) es crucial para la señalización celular.
  • El número y la disposición de los fosfatos en los GPCR influyen en los efectos mediados por el arresto.
  • Comprender estos patrones de fosforilación es clave para descifrar las vías de señalización.

Objetivo del estudio:

  • Investigar cómo los patrones de fosforilación de GPCR influyen en la unión y conformación de la arrestina.
  • Para aclarar los mecanismos moleculares que subyacen a estas interacciones.
  • Para proporcionar información estructural sobre el arresto de GPCR

Principales métodos:

  • Simulaciones moleculares a nivel atómico.
  • Espectroscopia dirigida al sitio.
  • Modelado computacional de las interacciones GPCR-arrestina.

Principales resultados:

  • Los patrones de fosforilación de las GPCR, no sólo el número total de fosfatos, dictan los cambios de unión y conformación.
  • Los diferentes arreglos de fosforilación pueden dar lugar a distintas conformaciones de arrestin y resultados de señalización.
  • Los sitios específicos de fosforilación ejercen efectos opuestos en el comportamiento de la arrestina.

Conclusiones:

  • La disposición de los fosfatos en los GPCR actúa como un