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Canal de iones sintéticos formados por anfifiles multibloqueados con doble sensibilidad anisotrópica
Ryo Sasaki1, Kohei Sato1, Kazuhito V Tabata2
1School of Life Science and Technology, Tokyo Institute of Technology, 4259, Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Journal of the American Chemical Society
|January 14, 2021
Resumen
Los investigadores desarrollaron un canal iónico sintético que imita los canales naturales. Este nuevo canal de iones supramolecular
Área de la Ciencia:
- Química biomimética
- Química supramolecular
- Biofísica de las membranas
Sus antecedentes:
- Las proteínas transmembrana son cruciales para las funciones celulares.
- Los miméticos sintéticos de las proteínas transmembrana ayudan a comprender los eventos biológicos.
- Los canales iónicos naturales exhiben un comportamiento anisotrópico y multiestímulo.
Objetivo del estudio:
- Desarrollar un canal iónico sintético inspirado en los canales iónicos naturales.
- Para investigar las propiedades de transporte de iones controladas por voltaje.
- Para lograr una respuesta anisotrópica a los estímulos duales en un canal iónico sintético.
Principales métodos:
- Incorporación de un anfífilo de varios bloques (V) en las membranas de las dos capas lipídicas.
- Investigación de las propiedades de transporte de iones bajo tensión variable.
- Utilizando la espectroscopia de emisión microscópica para estudiar los cambios de conformación molecular.
- El cambio reversible del transporte de iones mediante el uso de (R) propranolol y β-ciclodextrina.
Principales resultados:
- El anfífilo sintético V formó un canal iónico supramolecular en las bicapas lipídicas.
- El transporte de iones a través del canal V era controlable por la polaridad y la amplitud del voltaje.
- Cambios conformacionales inducidos por el voltaje en la molécula V.
- El transporte de iones fue cambiado reversiblemente por (R) - propranolol y β- ciclodextrina.
Conclusiones:
- Se desarrolló un canal de iones supramolecular sintético con transporte de iones controlado por voltaje.
- El canal exhibe una respuesta anisotrópica a los estímulos duales.
- Este trabajo proporciona información sobre el diseño de canales de iones sintéticos sofisticados.
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