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activación sensible al pH del sistema de regulación del pH intracelular en los axones de los calamares por el

W F Boron¹, E Hogan, J M Russell

¹Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.

|March 17, 1988

Resumen

La regulación del pH intracelular es vital para la función celular. Este estudio muestra que el ATP activa el intercambiador cloruro-bicarbonato dependiente del sodio, crucial para el equilibrio del pH, en lugar de ser consumido por él.

Área de la Ciencia:

Fisiología celular Fisiología celular.
Transporte de la membrana Transporte de la membrana
La bioquímica es la bioquímica.

Sus antecedentes:

La regulación del pH intracelular (pHi) es crítica para la función y activación celular.
El intercambio cloruro-bicarbonato dependiente del sodio es un regulador primario de pHi en muchos tipos de células.
El requisito de ATP para este intercambiador en los axones de los calamares sigue siendo mecánicamente incierto.

Objetivo del estudio:

Aclarar el papel del ATP en la función del intercambiador cloruro-bicarbonato dependiente del sodio.
Para diferenciar entre la hidrólisis de ATP (hipótesis de la bomba) y la activación de ATP (hipótesis de activación) para la función del transportador.

Principales métodos:

La diálisis de los axones de los calamares con el análogo no hidrolizable del ATP, ATP-gamma-S.
Investigación del papel del ATP en la regulación del pH mediada por el intercambiador Cl-HCO3- dependiente de Na+.

Principales resultados:

El estudio descartó la hipótesis de la bomba, demostrando que el ATP no se consume estequiométricamente.
Los resultados indican que se requiere ATP para la activación dependiente del pH del intercambiador.
La activación puede ocurrir a través de la fosforilación o la unión directa de ATP al transportador o un activador.

Conclusiones:

El ATP es esencial para activar el intercambiador cloruro-bicarbonato dependiente del sodio, no para el suministro directo de energía a través de la hidrólisis.
Los hallazgos aclaran un mecanismo clave en la homeostasis del pH celular y en los procesos de activación celular.

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