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Updated: Aug 15, 2026
17:27
Preparation of Aplysia Sensory-motor Neuronal Cell Cultures
Published on: June 8, 2009
Inhibición heterosináptica a largo plazo en Aplysia
P G Montarolo1, E R Kandel, S Schacher
1Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York.
Nature
|May 12, 1988
Resumen
El péptido Fe-Met-Arg-Fe-amida (FMRFamida) induce la depresión sináptica a largo plazo en Aplysia.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- La neurociencia celular es la neurociencia celular.
- La plasticidad sináptica.
Sus antecedentes:
- La transmisión sináptica en el reflejo de retirada de branquias de Aplysia exhibe modulación a corto y largo plazo.
- La depresión sináptica a corto plazo, de duración de minutos, es inducida por Phe-Met-Arg-Phe-amida (FMRFamida) a través de la vía de la lipoxigenasa del ácido araquidónico.
Objetivo del estudio:
- Para investigar la inducción de la depresión sináptica a largo plazo (LTD) en el reflejo de retirada de las branquias de Aplysia.
- Para determinar si la FMRFamida puede inducir LTD y comparar sus características con la depresión a corto plazo y otras vías moduladoras.
Principales métodos:
- Se utilizó un cultivo celular de Aplysia para estudiar la conexión monosináptica sensorial-motora.
- Se aplicó FMRFamide repetidamente durante un período de 2 horas para inducir cambios a largo plazo.
- Investigó el efecto de la anisomicina, un inhibidor de la síntesis de proteínas, en la depresión inducida por FMRFamida.
Principales resultados:
- Cinco aplicaciones de FMRFamide durante 2 horas indujeron depresión sináptica a largo plazo que duró 24 horas.
- La LTD inducida por FMRFamide fue específica del transmisor; la dopamina y la estimulación de baja frecuencia no indujeron LTD.
- La depresión a largo plazo, a diferencia de la depresión a corto plazo, fue bloqueada por la anisomicina, lo que indica un requisito para la síntesis de proteínas.
Conclusiones:
- La depresión sináptica a largo plazo en Aplysia, similar a la facilitación a largo plazo, requiere la síntesis de proteínas.
- La depresión heterosináptica, como la facilitación, existe tanto en formas a corto como a largo plazo, siendo esta última dependiente de los productos genéticos.
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