Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.5K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.5K
Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

3.1K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
3.1K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.2K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.2K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.1K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.1K
What are Membranes?01:54

What are Membranes?

182.3K
A key characteristic of life is the ability to separate the external environment from the internal space. To do this, cells have evolved semi-permeable membranes that regulate the passage of biological molecules. Additionally, the cell membrane defines a cell’s shape and interactions with the external environment. Eukaryotic cell membranes also serve to compartmentalize the internal space into organelles, including the endomembrane structures of the nucleus, endoplasmic reticulum and...
182.3K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.7K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.7K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Plasma-SELEX for Acute Myocardial Infarction Biomarker Discovery and Diagnosis.

Analytical chemistry·2026
Same author

Reprogramming Aromatic Camptothecins into TOP1 Degraders via Synergistic Hydrophobic Tagging and Supramolecular Assembly.

Journal of the American Chemical Society·2026
Same author

Author Correction: DNA nanodevices detect an acidic nanolayer on the lysosomal surface.

Nature cell biology·2026
Same author

Receptor-Tethered Cytosolic Modulators Enable Spatial Control of Cell Signaling Specificity.

ACS nano·2026
Same author

Covalent Photo-Aptamer Tagging (CPAT) for Single-Cell Multiplexing and Chemical Transcriptomic Screens.

Analytical chemistry·2026
Same author

Modular nucleic acid-based construct for delivery of immunostimulatory agonists and oncogene-silencing oligonucleotides in tumours.

Nature biomedical engineering·2026

Video Experimental Relacionado

Updated: Nov 12, 2025

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems
07:10

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Published on: February 23, 2024

1.4K

Mímico dinámico basado en ADN de proteínas de membrana para la programación de interacciones celulares adaptativas

Jin Li1,2, Kanyu Xun2, Liyan Zheng2

  • 1The Cancer Hospital of the University of Chinese Academy of Sciences, Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.

Journal of the American Chemical Society
|March 18, 2021
PubMed
Resumen

Los investigadores diseñaron una nanoarquitectura de ADN de la superficie celular que imita las proteínas dinámicas de la membrana. Este sistema permite un control preciso de las interacciones celulares y la ingeniería celular terapéutica en respuesta a las señales ambientales.

Más Videos Relacionados

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy
12:24

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Published on: September 29, 2016

7.2K
Assembly and Characterization of Biomolecular Memristors Consisting of Ion Channel-doped Lipid Membranes
08:07

Assembly and Characterization of Biomolecular Memristors Consisting of Ion Channel-doped Lipid Membranes

Published on: March 9, 2019

8.1K

Videos de Experimentos Relacionados

Last Updated: Nov 12, 2025

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems
07:10

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Published on: February 23, 2024

1.4K
Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy
12:24

Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy

Published on: September 29, 2016

7.2K
Assembly and Characterization of Biomolecular Memristors Consisting of Ion Channel-doped Lipid Membranes
08:07

Assembly and Characterization of Biomolecular Memristors Consisting of Ion Channel-doped Lipid Membranes

Published on: March 9, 2019

8.1K

Área de la Ciencia:

  • Biotecnología
  • Biología sintética
  • Ingeniería celular

Sus antecedentes:

  • Las membranas celulares regulan dinámicamente la expresión y conformación de proteínas para sentir y responder al entorno.
  • Los métodos existentes para manipular las interacciones celulares a menudo carecen de respuesta dinámica a los estímulos externos.

Objetivo del estudio:

  • Para diseñar una nueva nanoarquitectura de superficie celular que imite el comportamiento dinámico de las proteínas de la membrana.
  • Para permitir el ensamblaje de ADN iniciado por reconocimiento molecular para interacciones celulares controladas.
  • Desarrollar una plataforma para diseñar células terapéuticas con capacidades de detección y respuesta personalizadas.

Principales métodos:

  • Desarrollo de una nanoarquitectura de ADN anclada en la membrana.
  • Utilizando el reconocimiento molecular para el ensamblaje de ADN desencadenado por señales de respuesta celular.
  • Demostrar una activación específica por estímulos externos.
  • Montaje de módulos funcionales en la membrana celular para unirse y matar de forma selectiva.

Principales resultados:

  • La nanoarquitectura de ADN diseñada imita con éxito el comportamiento dinámico de las proteínas de la membrana.
  • El sistema se activa específicamente por señales de respuesta celular y estímulos externos.
  • Se ensamblaron módulos funcionales en la membrana, lo que permite la unión y la muerte específicas del tipo de célula.
  • La plataforma demostró la capacidad de equipar a las células con vías de detección y respuesta personalizadas.

Conclusiones:

  • La nanoarquitectura de ADN de superficie celular desarrollada ofrece un nuevo enfoque para imitar las funciones dinámicas de las proteínas de la membrana.
  • Este sistema proporciona una plataforma versátil para diseñar células terapéuticas con capacidades de detección y respuesta ambientales a medida.
  • Los hallazgos presentan un nuevo paradigma para el avance de las terapias basadas en células a través de la biología sintética.