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Generación quimioenzimática de membranas fosfolípidas mediadas por la síntesis de ácidos grasos tipo I

  • 0Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.
Journal of the American Chemical Society +

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12 de mayo de 2021

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12 de mayo de 2021

Ver abstracta en PubMed

Resumen

Este resumen es generado por máquina.

Los investigadores crearon membranas lipídicas artificiales a partir de bloques de construcción básicos utilizando un nuevo método quimioenzimático. Este avance permite la formación <i>de novo</i> de vesículas unidas a la membrana a partir del acetil-CoA y el malonil-CoA.

Área De La Ciencia

  • Biología sintética
  • La bioquímica
  • Ciencias de los materiales

Sus Antecedentes

  • La formación de membrana lipídica es crucial para las células sintéticas, pero es difícil de replicar in vitro.
  • La síntesis natural de fosfolípidos implica vías enzimáticas complejas difíciles de reconstituir.
  • Los métodos actuales luchan por construir membranas a partir de precursores metabólicos simples.

Objetivo Del Estudio

  • Desarrollar una estrategia quimioenzimática para la generación de membrana lipídica de novo.
  • Para reconstituir la síntesis de membrana de fosfolípidos a partir de acetil-CoA y malonil-CoA.
  • Para crear una ruta biomimética para la auto-ensamblaje de las vesículas en la membrana.

Principales Métodos

  • Se utilizó una síntesis de ácidos grasos bacterianos solubles (cgFAS I) para la síntesis in situ de palmitoil-CoA.
  • Se utiliza la ligadura química nativa (LNC) para la reacción entre el derivado de ácido graso y el lisofosfolípido.
  • Autoensamblaje de amidofosfolípidos no canónicos en vesículas.

Principales Resultados

  • Se ha sintetizado con éxito palmitoyl-CoA a partir de acetil-CoA y malonyl-CoA.
  • Amidofosfolípidos no canónicos generados a través de NCL.
  • Se observa el autoensamblaje en vesículas de tamaño micrométrico.

Conclusiones

  • Se demostró la primera reconstitución de la formación de membrana fosfolípida directamente a partir de acetil-CoA y malonil-CoA.
  • Síntesis enzimática combinada con bioconjugación para una eficiente formación de vesículas.
  • Estableció un enfoque biomimético para la generación de membranas celulares sintéticas de novo.

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