Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.4K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.4K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.4K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.4K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

24.8K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
24.8K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

4.8K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.8K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Embryonic Stem Cells00:57

Embryonic Stem Cells

4.2K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
4.2K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Spatiotemporal heterogeneity of recurrence and predictors after radical surgery for pancreatic ductal adenocarcinoma: a retrospective observational study.

Surgical endoscopy·2026
Same author

Continuous modeling of primate embryogenesis from totipotency to early organogenesis.

Cell·2026
Same author

The mechanism of action and therapeutic potential of macrophages in osteoporosis: from polarization balance to targeted regulation.

Frontiers in immunology·2026
Same author

Learning curve of laparoscopic duodenum-preserving total pancreatic head resection: a single-center dual CUSUM and risk-adjusted CUSUM analysis.

Surgical endoscopy·2026
Same author

Analysis and application of microbiota in fermentation pit muds used for Chinese strong-flavor liquor production.

AMB Express·2026
Same author

Comparative Severe Complications After Laparoscopic Pancreatoduodenectomy in PDAC vs Non-PDAC Periampullary Cancer: A Retrospective Cohort Study.

Journal of surgical oncology·2026

Video Experimental Relacionado

Updated: Nov 5, 2025

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K

Células madre totipotentes de ratón capturadas y mantenidas mediante represión espliceosómica

Hui Shen1, Min Yang2, Shiyu Li1

  • 1MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

Cell
|May 15, 2021
PubMed
Resumen

Los investigadores desarrollaron un método para crear células madre totipotentes (CET) in vitro. Al inhibir el empalme en células madre embrionarias de ratón (ESC), generaron TSC con pleno potencial de desarrollo, avanzando en la investigación de células madre.

Palabras clave:
Las quimerasembrionariocélulas madre embrionariasextraembrionarioPladienolida B y sus derivadosPluripotenteEspliceosomael empalmeTotipotente también.El transcriptoma

Más Videos Relacionados

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

12.5K
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

7.2K

Videos de Experimentos Relacionados

Last Updated: Nov 5, 2025

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

12.5K
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

7.2K

Área de la Ciencia:

  • Biología del desarrollo
  • Biología de las células madre
  • Biología molecular

Sus antecedentes:

  • El establecimiento de cultivos in vitro de células totipotentes comparables a los blastómeros in vivo ha sido un desafío significativo.
  • Las células madre embrionarias (ESC) poseen pluripotencia pero carecen del potencial de desarrollo completo de los blastómeros tempranos.

Objetivo del estudio:

  • Investigar el papel de la actividad espliceosómica en la regulación de la transición de la pluripotencia a la totipotencia.
  • Desarrollar un método para el cultivo in vitro estable de células totipotentes.

Principales métodos:

  • Se utilizó pladienolida B, un inhibidor de empalme, para modular la actividad espliceosómica en células ESC de ratón.
  • Se utilizaron ensayos quiméricos en ratones y secuenciación de ARN de una sola célula (sc-RNA seq) para evaluar el potencial de desarrollo y las características moleculares.
  • Se analizaron patrones de empalme de genes pluripotentes y totipotentes.

Principales resultados:

  • Se obtiene un cultivo in vitro estable de células similares a los blastómeros totipotentes (TBLC) de células ESC de ratón.
  • Los TBLC demostraron una sólida capacidad de desarrollo bidireccional, generando linajes tanto embrionarios como extraembrionarios.
  • La represión espliceosómica inhibió el empalme de genes pluripotentes mientras activaba genes totipotentes con intrones cortos.

Conclusiones:

  • La represión espliceosómica es un mecanismo clave que impulsa la transición de las ESC a un estado totipotente.
  • El método desarrollado permite la captura y el mantenimiento de células madre totipotentes in vitro.
  • Este avance ofrece nuevas posibilidades para el estudio del desarrollo embrionario temprano y la medicina regenerativa.