Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Gene Conversion02:08

Gene Conversion

10.2K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.2K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.0K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.0K
Homologous Recombination02:31

Homologous Recombination

57.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
57.7K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

13.3K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
13.3K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

3.7K
3.7K
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

63.1K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
63.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

A multi-species toolkit of TOP2 hypercleavage mutants for studying topoisomerase II-mediated DNA damage.

bioRxiv : the preprint server for biology·2026
Same author

Symptom-Based Dosing for Neonatal Opioid Withdrawal: The OPTimize NOW Randomized Clinical Trial.

JAMA·2026
Same author

Examining the 'Consumer Repair Journey' and Business Intervention Opportunities to Enhance Customer Experience and Satisfaction.

Circular economy and sustainability·2026
Same author

Temporal trends in outcomes for infants with neonatal opioid withdrawal managed with the Finnegan scoring tool: Insights from the INFORM NOW Study.

Journal of perinatology : official journal of the California Perinatal Association·2026
Same author

Recruitment of Mre11 to recombination sites during meiosis.

Nature communications·2026
Same author

MRE11 suppresses germline mutagenesis at meiotic double-strand breaks in mice.

bioRxiv : the preprint server for biology·2026

Video Experimental Relacionado

Updated: Nov 2, 2025

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI

Published on: September 24, 2015

10.3K

El corte concertado por Spo11 ilumina la mecánica de ruptura del ADN meiótico

Dominic Johnson1, Margaret Crawford1, Tim Cooper1

  • 1Genome Damage and Stability Centre, University of Sussex, Brighton, UK.

Nature
|June 10, 2021
PubMed
Resumen

Los investigadores descubrieron que la proteína Spo11 crea múltiples rupturas de doble cadena de ADN (DSB) durante la meiosis, formando lagunas en el ADN. Este hallazgo revisa nuestra comprensión de la recombinación meiótica y los mecanismos de reparación de brechas.

Más Videos Relacionados

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication
05:55

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication

Published on: August 23, 2024

770
Flow Cytometry Purification of Mouse Meiotic Cells
10:43

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

17.9K

Videos de Experimentos Relacionados

Last Updated: Nov 2, 2025

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI

Published on: September 24, 2015

10.3K
Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication
05:55

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication

Published on: August 23, 2024

770
Flow Cytometry Purification of Mouse Meiotic Cells
10:43

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

17.9K

Área de la Ciencia:

  • Biología molecular
  • La genética
  • Biología celular

Sus antecedentes:

  • La recombinación genética durante la meiosis es crucial para la segregación cromosómica precisa.
  • Las rupturas de doble cadena de ADN (DSB), iniciadas por la proteína similar a la topoisomerasa Spo11, son intermedios clave en la recombinación meiótica.
  • El mecanismo preciso por el cual Spo11 cataliza la escisión del ADN y su compromiso con el sustrato sigue siendo poco conocido.

Objetivo del estudio:

  • Investigar el mecanismo detallado de la ruptura de doble cadena de ADN mediada por Spo11 (DSB) durante la meiosis.
  • Caracterizar los nuevos patrones de formación de DSB más allá de los cortes individuales.
  • Para aclarar las implicaciones de estos hallazgos para la recombinación meiótica y la reparación del ADN.

Principales métodos:

  • Utilizó Saccharomyces cerevisiae como un organismo modelo.
  • Se empleó la secuenciación profunda de la progenie meiótica para analizar los eventos de recombinación.
  • Se han realizado ensayos de unión al ADN in vitro con el complejo de núcleo Spo11.

Principales resultados:

  • Identificó la formación de "dobles cortes", que son hiperlocalizados, concertados Spo11 DSB separados por 33 a más de 100 pares de bases.
  • Se observó que las longitudes de estos dobles cortes exhiben una periodicidad conservada de 10,5 pares de bases en levadura y ratones.
  • Proporcionó evidencia que apoya un modelo en el que las moléculas adyacentes de Spo11 tienen orientaciones fijas en relación con la hélice de ADN.
  • Se detectaron cicatrices de recombinación consistentes con la reparación iniciada por las brechas de ADN generadas por Spo11.

Conclusiones:

  • Spo11 puede generar DSBs adyacentes, creando brechas en el ADN durante la meiosis.
  • La periodicidad de los cortes dobles sugiere una orientación restringida de Spo11 en el ADN.
  • Estos hallazgos amplían el concepto de reparación de brechas en la meiosis para incluir brechas de ADN iniciadas por Spo11, revisando los modelos actuales de la mecánica de formación de DSB.