Video Experimental Relacionado
Updated: Oct 20, 2025
11:37
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
Published on: July 28, 2017
19.3K
Una plataforma P(V para la síntesis de oligonucleótidos
Yazhong Huang1,2, Kyle W Knouse3,2, Shenjie Qiu2,4
1Small Molecule Drug Discovery, Bristol Myers Squibb, Cambridge, MA 02142, USA.
Science (New York, N.Y.)
|September 13, 2021
Resumen
Los investigadores desarrollaron una nueva plataforma basada en P ((V) para crear diversos enlaces de fosfato modificados en oligonucleótidos terapéuticos. Este método flexible simplifica la síntesis de terapias basadas en genes, mejorando la accesibilidad y las propiedades.
Área de la Ciencia:
- Química de los oligonucleótidos
- Química medicinal
- Terapia génica
Sus antecedentes:
- Los oligonucleótidos terapéuticos a menudo contienen enlaces de fosfato modificados para mejorar las propiedades biológicas y físicas.
- Los métodos de síntesis actuales, como la química de la fosforamidita, pueden ser un desafío para acceder a diversos enlaces modificados.
Objetivo del estudio:
- Desarrollar una plataforma flexible y eficiente para la síntesis de varios enlaces de fosfato en oligonucleótidos.
- Permitir la fácil instalación de diversos enlaces, incluidos los tiofosfatos, los fosfodiéster y los fosforodithioatos.
Principales métodos:
- Para la síntesis de oligonucleótidos se empleó una nueva plataforma química basada en P{\displaystyle P}
- Se utilizaron reactivos fácilmente accesibles y un protocolo de acoplamiento estandarizado.
- La plataforma incluye tiofosfatos estereodefinidos o racémicos (PS) junto con enlaces de fosfodiéster (PO2) y fosforodiitioato (PS2) nativos.
Principales resultados:
- La plataforma basada en P (V) demostró flexibilidad en la instalación de una amplia gama de enlaces de fosfato.
- El método sintetizó con éxito ADN y otros polímeros de nucleótidos modificados con diversos enlaces.
- Se utilizaron reactivos P (V) estables preparados de manera sostenible según un protocolo estandarizado.
Conclusiones:
- La plataforma desarrollada basada en P(V) ofrece un enfoque eficiente y versátil para la síntesis de oligonucleótidos.
- Este método facilita la creación de diversos enlaces de fosfato modificados cruciales para el avance de las terapias basadas en genes.
- La plataforma simplifica el acceso a estructuras complejas de oligonucleótidos, acelerando potencialmente el desarrollo terapéutico.
Videos de Conceptos Relacionados
01:28ATP and Macromolecule Synthesis
6.3K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
6.3K
01:28Biosynthesis of Nucleic Acids
338
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
338
01:59Lagging Strand Synthesis
55.5K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
55.5K
03:01The Replisome
36.1K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
36.1K
02:36Transfer RNA Synthesis
12.4K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
12.4K