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Modulación parasitaria del desarrollo del huésped por degradación de proteínas independiente de la ubiquitina

Weijie Huang¹, Allyson M MacLean¹, Akiko Sugio¹

¹Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.

|September 18, 2021

Resumen

Los fitoplasmas patógenos de plantas utilizan efectores SAP05 para manipular el desarrollo del huésped. Estas proteínas secuestran el receptor de ubiquitina de la planta RPN10, prolongando la vida útil del huésped e induciendo un crecimiento anormal para favorecer la transmisión de parásitos.

Área de la Ciencia:

Patología vegetal
Biología molecular
Parasitología

Sus antecedentes:

Los parásitos obligados pueden alterar significativamente los fenotipos del huésped para mejorar la transmisión.
Los mecanismos moleculares detrás de estas manipulaciones de huéspedes por los patógenos vegetales vectoriales de insectos son poco conocidos.

Objetivo del estudio:

Aclarar cómo las proteínas efectoras SAP05 de los fitoplasmas controlan el desarrollo de las plantas.
Identificar los objetivos y mecanismos de acogida explotados por SAP05.

Principales métodos:

Investigó la función de las proteínas efectoras SAP05 en las plantas huésped.
Analizó la interacción entre SAP05 y las proteínas reguladoras del huésped.
Utilizó técnicas moleculares para estudiar las vías de degradación de proteínas y la unión al receptor.

Principales resultados:

Los efectores SAP05 inducen la extensión de la vida del huésped y la proliferación de las brujas.
SAP05 media la degradación de los reguladores del desarrollo SPL y GATA.
Esta degradación secuestra el receptor de ubiquitina de la planta RPN10, independientemente de la ubiquitinación.
SAP05 se dirige específicamente a la planta RPN10, no al insecto vector RPN10.

Conclusiones:

Una proteína efector única, SAP05, orquesta múltiples cambios de desarrollo en las plantas huésped.
Los fitoplasmas explotan el sistema de ubiquitina de la planta a través de RPN10 para manipular el desarrollo del huésped en su beneficio.
La comprensión de este mecanismo ofrece información sobre las interacciones entre plantas y parásitos y la evolución de los efectores.

Palabras clave:

transición de la fase de desarrollo Vectores de insectos Micoplasma y sus derivados bacterias no cultivables El floema patógeno de las plantas degradación proteica dirigida Sistema ubiquitino-proteasómico Las bacterias que colonizan la vasculatura Planta zombi

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