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Updated: May 2, 2026

Protein Transfection of Mouse Lung

Protein Transfection of Mouse Lung

Published on: May 15, 2013

La expresión en los fibroblastos de rata de un factor de crecimiento humano transformador alfa cDNA da como resultado

A Rosenthal, P B Lindquist, T S Bringman

|July 18, 1986

Resumen

La transformación de la producción de factor de crecimiento alfa (TGF-alfa) por las células de Rat-1 indujo la transformación maligna, lo que condujo a la formación de tumores. Los anticuerpos neutralizantes bloquearon este efecto promotor del tumor, confirmando el TGF-alfa.

Área de la Ciencia:

Biología celular Biología celular.
Biología Molecular Biología Molecular
Oncología Oncología.

Sus antecedentes:

Los factores de crecimiento, como el factor de crecimiento transformador alfa (TGF-alfa), pueden impulsar la proliferación celular y potencialmente conducir a la transformación maligna.
Las células tumorales con frecuencia secretan TGF-alfa, apoyando el modelo de estimulación autocrina en el desarrollo del cáncer.

Objetivo del estudio:

Para investigar directamente el papel del TGF-alfa en la tumorigenicidad celular.
Para determinar si la expresión de TGF-alfa es suficiente para inducir la transformación maligna en las células no transformadas.

Principales métodos:

Introducción de un vector de expresión de TGF-alfa cDNA humano en las células no transformadas del fibroblasto de rata Fischer (Rat-1).
Evaluación del crecimiento dependiente del anclaje y la formación de tumores en ratones desnudos.
Inhibición de la formación de colonias en el agar blando mediante el uso de anticuerpos monoclonales anti-humanos TGF-alfa.

Principales resultados:

Las células rat-1 diseñadas para sintetizar y secretar TGF-alfa humano exhibieron pérdida de crecimiento dependiente del anclaje.
Estas células Rat-1 que expresan TGF-alfa formaron tumores en ratones desnudos, lo que indica una tumorigenicidad inducida.
Los anticuerpos monoclonales antihumanos TGF-alfa impidieron efectivamente la formación de colonias en el agar blando por las células que expresan TGF-alfa.

Conclusiones:

La producción autocrina de TGF-alfa es suficiente para inducir la transformación maligna y la tumorigenicidad en los fibroblastos no transformados.
Dirigirse a la señalización del TGF-alfa con anticuerpos puede inhibir los efectos promotores de tumores del TGF-alfa.

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Transcription

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

Transcription Factors

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Forced Transdifferentiation

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...