Video Experimental Relacionado
Updated: Jul 14, 2026
14:23
Recombineering Homologous Recombination Constructs in Drosophila
Published on: July 13, 2013
Los segmentos de genes de la región variable del receptor de células T introducidos se recombinan en células pre-B:
Cell
|January 31, 1986
Resumen
Una recombinasa común puede ensamblar segmentos de genes variables en células B y T, con una especificidad reguladora de accesibilidad. Se introdujeron segmentos de genes del receptor de células T (TCR) reorganizados en células pre-B, lo que sugiere un papel para los potenciadores de inmunoglobulina en la recombinación.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- El ensamblaje de segmentos de genes de región variable en los linfocitos B y T es crucial para la inmunidad adaptativa.
- Una hipótesis sugiere que una sola enzima recombinasa media estos eventos de recombinación V(D) J.
- Se propone controlar la especificidad mediante la regulación de la accesibilidad de segmentos genéticos específicos.
Objetivo del estudio:
- Para investigar el papel de la accesibilidad del segmento genético en la recombinación V(D) J.
- Para probar si los segmentos del gen del receptor de células T (TCR) pueden ser reorganizados por la maquinaria presente en las células B.
- Para explorar la función de los potenciadores de inmunoglobulina (Ig) en el proceso de recombinación.
Principales métodos:
- Transfección de segmentos de genes de la región variable del receptor de células T (TCR) "accesible" en una línea celular pre-B.
- Análisis de la frecuencia de recombinación y las características de los segmentos de genes TCR introducidos.
- Comparación de la recombinación del segmento del gen TCR con la recombinación del segmento del gen de la inmunoglobulina (Ig) endógena.
Principales resultados:
- Introducidos segmentos del gen TCR reorganizados con frecuencia en la línea celular pre-B.
- Las características de recombinación de los segmentos de TCR introducidos se asemejaban a las de los segmentos de genes Ig.
- Los segmentos endógenos del gen TCR, normalmente inaccesibles, no se reorganizaron.
Conclusiones:
- La accesibilidad del segmento génico es un factor clave en la especificidad de la recombinación V(D) J.
- Los potenciadores transcripcionales de la inmunoglobulina (Ig) pueden funcionar como "potenciadores recombinantes".
- El estudio proporciona información sobre la inserción de la región N, la unión aberrante y los requisitos de la secuencia de recombinación.
Más Videos Relacionados
Videos de Conceptos Relacionados
00:57Viral Recombination
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
02:31Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
02:53Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
02:31Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
01:30Crossing Over
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...
01:28Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...