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Videos de Conceptos Relacionados

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Video Experimental Relacionado

Updated: May 8, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Los exones 5' alternativos en el ARNm c-abla mRNA.

Y Ben-Neriah, A Bernards, M Paskind

    Cell
    |February 28, 1986
    PubMed
    Resumen

    El proto-oncogén celular abl, una proteína-tirosina quinasa, muestra variados tamaños de ARNm debido al empalme alternativo. Este empalme ocurre en el mismo sitio involucrado en la translocación del cromosoma de Filadelfia.

    Área de la Ciencia:

    • Biología Molecular Biología Molecular
    • La oncogénesis es la oncogénesis.
    • Expresión génica de la expresión génica.

    Sus antecedentes:

    • El proto-oncogén celular abl codifica una proteína tirosina kinasa.
    • Este gen se expresa en varios tipos de células, por lo general como dos o tres distintas variantes de tamaño de ARNm.

    Objetivo del estudio:

    • Para investigar la diversidad estructural de los c-abl cDNAs de ratón.
    • Comprender los mecanismos que generan la heterogeneidad en el ARNm c-abl.
    • Para explorar la relación entre el empalme c-abl y las transformaciones oncogénicas.

    Principales métodos:

    • Clonado de cuatro tipos de ADN complementario (ADNc) de ratón c-abl a partir de células linfoides 70Z/3.
    • Análisis de las secuencias 5' de los cDNA clonados para determinar las secuencias de aminoácidos N-terminales predichas.

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    Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
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  • Comparación de las secuencias de c-abl cDNA con las secuencias virales abl (v-abl).

    • Principales resultados:

    • Se identificaron cuatro distintos c-abl cDNA de ratón, cada uno con secuencias únicas de 5' que codifican las regiones N-terminales predichas de 20-45 aminoácidos.
    • Un cDNA compartió una secuencia met-gly-gln N-terminal con el extremo gag N de v-abl.
    • La heterogeneidad 5' observada en los c-abl cDNA se atribuye a la adición alternativa de 5' exones a un conjunto común de 3' exones.
    • Se encontró que el empalme alternativo ocurre en el mismo sitio donde las secuencias BCR se unen a las secuencias ABL en la translocación del cromosoma de Filadelfia.

    Conclusiones:

    • El proto-oncogén celular abl exhibe una diversidad estructural significativa a nivel de ARNm.
    • El empalme alternativo es un mecanismo clave que genera diferentes formas de c-abl.
    • El sitio de empalme involucrado en la heterogeneidad c-abl se conserva en el contexto de translocaciones oncogénicas como el cromosoma de Filadelfia.