Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

Transfer RNA Synthesis

Transfer RNA Synthesis

No description available

Translation

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...

Translation

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

pre-mRNA Processing

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Quantitative functional outcomes of the conservative management of partial segmental thrombosis of the corpus cavernosum.

Annals of the Royal College of Surgeons of England·2015

Same author

The cytoplasmic and nuclear populations of the eukaryote tRNA-isopentenyl transferase have distinct functions with implications in human cancer.

Gene·2014

Same author

Division of labor among the yeast Sol proteins implicated in tRNA nuclear export and carbohydrate metabolism.

Genetics·2004

Same author

The outcome of drug smuggling by 'body packers'--the British experience.

Annals of the Royal College of Surgeons of England·2002

Same author

Maf1p, a negative effector of RNA polymerase III in Saccharomyces cerevisiae.

Molecular and cellular biology·2001

Same author

Role of nuclear pools of aminoacyl-tRNA synthetases in tRNA nuclear export.

Molecular biology of the cell·2001

Search research articles

Video Experimental Relacionado

Updated: Mar 8, 2026

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Published on: July 20, 2019

Un mutante de la levadura que acumula los tRNA precursores.

A K Hopper, F Banks

|June 1, 1978

Resumen

El mutante de levadura ts136 acumula nuevas especies de ARN, lo que sugiere un defecto en la biosíntesis y procesamiento del ARN de transferencia (ARNt). Este defecto nucleolítico también puede impedir el transporte del ARN mensajero (ARNm).

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética de la levadura Genética de la levadura
Procesamiento de ARN Procesamiento de ARN

Sus antecedentes:

Anteriormente se propuso que el mutante de levadura condicional ts136 fuera defectuoso en el transporte de ARNm nuclear a citoplasmático.
Este estudio investiga una hipótesis alternativa: un defecto potencial en la biosíntesis del tRNA.

Objetivo del estudio:

Para caracterizar el defecto de procesamiento de ARN en la levadura mutante ts136.6.
Para determinar si ts136 es defectuoso en la biosíntesis de tRNA.
Para explorar la relación entre el procesamiento de tRNA y el transporte de mRNA.

Principales métodos:

Análisis de las especies de ARN acumuladas por ts136 a temperaturas restrictivas utilizando electroforesis en gel de poliacrilamida.

Más Videos Relacionados

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 25, 2010

Videos de Experimentos Relacionados

Last Updated: Mar 8, 2026

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Published on: July 20, 2019

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 25, 2010

Estudios de hibridación utilizando plásmidos de E. coli que contienen genes de ARNt de levadura.

Evaluación del contenido de bases modificadas y metiladas en especies de ARN acumuladas.

Principales resultados:

ts136 acumula doce nuevas especies de ARN a la temperatura restrictiva.
Algunos ARN acumulados co-migran con los tRNA precursores e hibridan con los genes del tRNA.
Tres especies de ARN (1a, 1b, 1c) son lo suficientemente grandes para dos ARNt en tándem y carecen de bases modificadas.
Otros ARN acumulados (2-8) contienen bases típicas modificadas por ARNt y se hibridan con genes de ARNt.
La síntesis de ARN ribosomal también se ve afectada en ts136.

Conclusiones:

El mutante de levadura ts136 exhibe defectos tanto en la biosíntesis / procesamiento de tRNA como en la síntesis de ARN ribosomal.
Las especies de ARN acumuladas sugieren un defecto en una actividad nucleolítica esencial para el procesamiento de ARN.
Se supone que este defecto nucleolítico es un requisito previo para el transporte eficiente de ARN, lo que podría explicar el defecto de transporte de ARNm previamente observado.