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Transaminación criptográfica oxidativa de la hidroxinaftoquinona en la biosíntesis de productos naturales

Tomohiro Noguchi¹, Shota Isogai¹, Tohru Terada^1,2

¹Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JAPAN.

Journal of the American Chemical Society

|March 16, 2022

Resumen

Los investigadores descubrieron NphE, una nueva enzima dependiente del piridoxal 5'-fosfato (PLP). Esta enzima utiliza exclusivamente oxígeno (O2) tanto para la transaminación como para la oxidación, formando 8-amino-flaviolina (8-AF) como un intermediario clave en la biosíntesis de productos naturales.

Área de la Ciencia:

La bioquímica
Enzimología
Biosíntesis de productos naturales

Sus antecedentes:

Las enzimas dependientes del piridoxal 5-fosfato (PLP) son catalizadores cruciales, pero pocas utilizan oxígeno molecular (O2).
La biosíntesis de productos naturales complejos a menudo implica vías enzimáticas e intermedios únicos.

Objetivo del estudio:

Identificar y caracterizar una nueva enzima dependiente del PLP capaz de reacciones dependientes del oxígeno.
Para aclarar el papel de esta enzima y su producto en la biosíntesis de productos naturales meroterpenoides.

Principales métodos:

Ensayos de actividad enzimática
Análisis estructural utilizando AlphaFold v2.0 y simulaciones de dinámica molecular
Estudios in vivo y mutagénesis dirigida al sitio

Principales resultados:

NphE, una nueva enzima dependiente de PLP, cataliza tanto la transaminación como la oxidación dependiente de O2.
La enzima forma 8-amino-flaviolina (8-AF) a través de un intermediario quinonoide reactivo, con O2 reducido a H2O2.
El 8-AF se identifica como un intermediario común con un grupo amino críptico para la biosíntesis de la nafta.

Conclusiones:

NphE representa una clase única de enzimas dependientes de PLP con funciones catalíticas duales que involucran O2.
El descubrimiento de 8-AF como un intermediario biosintético amplía nuestra comprensión de la síntesis de productos naturales híbridos terpenoide-policetida.

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