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Biocatalizador multifuncional para la reducción conjugada y la aminación reductora
Thomas W Thorpe1, James R Marshall1, Vanessa Harawa1
1Department of Chemistry, University of Manchester, Manchester Institute of Biotechnology, Manchester, UK.
Nature
|April 7, 2022
Resumen
Una nueva enzima, EneIRED, sintetiza eficientemente los diastereómeros quirales de aminas, cruciales para los productos farmacéuticos y agroquímicos. Este biocatalizador utiliza un mecanismo no declarado para la síntesis asimétrica, simplificando la producción.
Área de la Ciencia:
- Biocatálisis y biología sintética
- Química orgánica
Sus antecedentes:
- Los diastereómeros de aminas quirales son esenciales en productos farmacéuticos y agroquímicos.
- Los métodos de síntesis actuales son a menudo ineficientes y de varios pasos.
- La síntesis asimétrica es crítica debido a las diferentes propiedades bioquímicas de los enantiómeros.
Objetivo del estudio:
- Caracterizar un nuevo biocatalizador multifuncional para la síntesis de aminas quirales.
- Para aclarar el mecanismo catalítico único de la enzima.
- Para explorar el potencial de la enzima en la síntesis asimétrica de aminas quirales complejas.
Principales métodos:
- Descubrimiento de enzimas a partir de una colección de imina reductasa metagenómica (IRED).
- Caracterización de los biocatalizadores, incluidos los estudios mecánicos y estructurales.
- Evaluación del alcance del sustrato de la enzima para el acoplamiento de carbonilos y aminas.
Principales resultados:
- Identificación y caracterización de EneIRED, un nuevo biocatalizador de Pseudomonas.
- EneIRED utiliza un mecanismo no informado que involucra la reducción de alqueno conjugado activado por aminas y la aminación reductora.
- La enzima produce eficientemente diastereómeros quirales de aminas con hasta tres estereocentros a partir de diversos sustratos.
Conclusiones:
- EneIRED ofrece una ruta eficiente hacia valiosos diastereómeros quirales de aminas.
- La familia IRED constituye una plataforma prometedora para el descubrimiento de nuevas actividades enzimáticas.
- Este trabajo avanza en la biología sintética y las aplicaciones de síntesis orgánica.
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