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Tioésteres de péptidos sensibles a las enzimas para dirigir el aparato de Golgi

Weiyi Tan1, Qiuxin Zhang1, Monica C Quiñones-Frías2

  • 1Department of Chemistry, Brandeis University, 415 South Street, Waltham, Massachusetts 02453, United States.

Journal of the American Chemical Society
|April 11, 2022
PubMed
Resumen

Los nuevos tioésteres peptídicos se dirigen al aparato de Golgi (GA) para el control del tráfico intracelular. Este método interrumpe el transporte de proteínas, lo que lleva a la muerte celular y ofrece una plataforma para controlar los destinos celulares.

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Área de la Ciencia:

  • Biología celular
  • Biología molecular
  • La bioquímica

Sus antecedentes:

  • El aparato de Golgi (GA) es fundamental para el tráfico intracelular.
  • La orientación selectiva de la AG presenta un desafío significativo en la biología celular.

Objetivo del estudio:

  • Desarrollar un nuevo método para atacar selectivamente el aparato Golgi.
  • Investigar el mecanismo de orientación y sus efectos posteriores en los procesos celulares.

Principales métodos:

  • Utilizó tioésteres peptídicos no convencionales como nuevos agentes dirigidos a la GA.
  • Mecanismos de captación celular investigados (endocitosis mediada por la caveolina, macropinocitosis) basados en la concentración.
  • Hidrólisis del tiopéptido analizado por medio de tioesterasas asociadas con GA y posterior dimerización y acumulación.
  • Se examinó el impacto de la acumulación de AG y ER en el tráfico de proteínas y la viabilidad celular.
  • Capacidad de orientación probada en varios tipos de células (humanas, murinas, *Drosophila*) y con estructuras peptídicas modificadas.

Principales resultados:

  • Los tioésteres peptídicos se dirigen eficazmente al aparato de Golgi en varios tipos de células.
  • La hidrólisis por tioesterasas conduce a la acumulación de tiopéptido en el GA y el ER.
  • La acumulación interrumpe el tráfico de proteínas e induce la muerte celular a través de múltiples vías.
  • La orientación de la GA influye en la distribución de proteínas como el NRAS.
  • Las modificaciones como el uso de l-difenilalanina mantienen la capacidad de orientación de GA.

Conclusiones:

  • Desarrolló una plataforma molecular sensible a la tioesterasa para el objetivo de GA.
  • Demostró la capacidad de controlar los destinos celulares mediante la manipulación de los niveles de proteínas GA y ER.
  • Este enfoque ofrece una nueva estrategia para la modulación del tráfico intracelular y el desarrollo terapéutico.