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Complejos de nucleoproteínas que regulan la expresión génica en la diferenciación de los adipocitos: participación
Cell
|June 19, 1987
Resumen
Los investigadores identificaron un elemento regulador (FSE2) en el gen del adipocito P2 (aP2). Los factores nucleares, incluido el c-fos, se unen a FSE2, regulando negativamente la actividad del gen aP2 durante la diferenciación de los adipocitos.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La diferenciación celular es la diferenciación celular.
- Reglamento genético Reglamento genético.
Sus antecedentes:
- La diferenciación de los adipocitos implica la activación transcripcional de genes específicos, como el gen del adipocito P2 (aP2), que codifica una proteína que se une a los lípidos.
- El gen aP2 posee un elemento regulador, denominado FSE2, ubicado aguas arriba de su sitio de inicio de transcripción.
Objetivo del estudio:
- Investigar el papel del elemento FSE2 en la regulación de la expresión génica aP2 durante la diferenciación de los adipocitos.
- Para identificar los factores nucleares que se unen a FSE2 y determinar su función en la regulación génica.
Principales métodos:
- Se utilizaron ensayos de retardación de gel para evaluar la unión específica de la secuencia y dependiente de la diferenciación de los factores nucleares al elemento FSE2.
- El análisis de eliminación de ensayos de transfección vinculados al promotor y los ensayos de competencia con elementos sintéticos de FSE2 se utilizaron para evaluar la función reguladora de FSE2.
- Los anticuerpos contra c-fos se utilizaron en ensayos de desplazamiento de gel y enlaces cruzados UV seguidos de inmunoprecipitación para determinar la participación de c-fos en el complejo de unión FSE2.
Principales resultados:
- Los factores nucleares se unen al elemento FSE2 de una manera específica de la secuencia y dependiente de la diferenciación.
- La evidencia sugiere que estos factores de acción trans actúan como reguladores negativos de la actividad del gen aP2 en los preadipocitos.
- Se descubrió que la proteína c-fos es un participante directo en el complejo de nucleoproteínas que se une a FSE2, como lo indica la interrupción del anticuerpo de la unión y la inmunoprecipitación específica.
Conclusiones:
- El elemento FSE2 es una región reguladora crítica para el gen aP2.
- Los factores nucleares, especialmente c-fos, se unen a FSE2 y reprimen la transcripción del gen aP2 en los preadipocitos.
- Este estudio aclara un nuevo mecanismo de regulación génica durante la diferenciación de los adipocitos que involucra c-fos y el elemento FSE2.
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