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Amiloidogénesis de la proteína del pico del SARS-CoV-2
Sofie Nyström1, Per Hammarström1
1Department of Physics, Chemistry and Biology, Linköping University, 58183 Linköping, Sweden.
Journal of the American Chemical Society
|May 17, 2022
Resumen
La proteína del pico del SARS-CoV-2 contiene secuencias propensas a formar fibrillas amiloides, que potencialmente contribuyen a la patogénesis del COVID-19. La actividad de la proteasa puede acelerar este proceso, ofreciendo información sobre los mecanismos de la enfermedad y el COVID-19 prolongado.
Área de la Ciencia:
- La bioquímica
- Biología molecular
- Virología
Sus antecedentes:
- La infección por SARS-CoV-2 presenta diversas enfermedades, incluidos problemas neurológicos y cardíacos.
- Las similitudes entre el COVID-19 y las enfermedades amiloides sugieren investigar la amiloidogenicidad de la proteína del pico del SARS-CoV-2.
Objetivo del estudio:
- Para investigar el potencial de la proteína de pico del SARS-CoV-2 (proteína S) para formar fibrillas amiloides.
- Explorar el papel de la endoproteólisis en la amiloidogénesis de la proteína S.
Principales métodos:
- Análisis de las fibrillas amiloides utilizando bibliotecas de péptidos y predicciones teóricas.
- Caracterización de péptidos de picos sintéticos utilizando el ensayo de tioflavina T (ThT), la unión roja del Congo y la microscopia electrónica.
- Incubación in vitro de la proteína S de longitud completa con la elastasa neutrófila (NE).
Principales resultados:
- Se identificaron siete secuencias amiloidogénicas en la proteína S.
- Tres péptidos de punta sintéticos cumplieron los criterios para las fibrillas amiloides (cinética, tinción, morfología).
- La elastasa de neutrófilos facilitó la formación de fibrillas tipo amiloide a partir de la proteína S y de segmentos amiloidogénicos expuestos.
Conclusiones:
- La proteína S del SARS-CoV-2 posee un potencial amiloidogénico intrínseco.
- La endoproteolisis por la neutrófila elastasa puede promover la amiloidogénesis de la proteína S, lo que sugiere un mecanismo molecular para la patogénesis de COVID-19.
- Comprender la amiloidogénesis de la proteína S puede ser crucial para abordar el COVID-19 y el COVID-19 prolongado.
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