Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

4.1K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.1K
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

14.6K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
14.6K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

7.0K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.0K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

6.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
6.3K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

10.1K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
10.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

mTOR Substrate Phosphorylation in Growth Control: An Update.

Cancers·2026
Same author

Multi-omics profiling reveals divergent biology and liver microenvironment in HCC of metastatic and de novo origin.

Molecular cancer·2026
Same author

Acid Versus Amide-Facts and Fallacies: A Case Study in Glycomimetic Ligand Design.

Molecules (Basel, Switzerland)·2025
Same author

Identification of transglutaminase 2 mediated polyaminated proteins in a hepatocellular cancer cell line.

Computational and structural biotechnology journal·2025
Same author

A multichaperone condensate enhances protein folding in the endoplasmic reticulum.

Nature cell biology·2025
Same author

Architecture and conformational dynamics of the BAM-SurA holo insertase complex.

Science advances·2025

Video Experimental Relacionado

Updated: Sep 23, 2025

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

19.2K

Fosforilación del sustrato mTOR en el control del crecimiento

Stefania Battaglioni1, Don Benjamin1, Matthias Wälchli1

  • 1Biozentrum, University of Basel, Spitalstrasse 41, 4056 Basel, Switzerland.

Cell
|May 17, 2022
PubMed
Resumen
Este resumen es generado por máquina.

El objetivo de la rapamicina (TOR) quinasa regula el crecimiento celular y el metabolismo. Los complejos TOR de mamíferos mTORC1 y mTORC2 fosforilan sustratos distintos utilizando un motivo común, a pesar de compartir una subunidad catalítica.

Más Videos Relacionados

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

287
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

8.8K

Videos de Experimentos Relacionados

Last Updated: Sep 23, 2025

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
08:04

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

19.2K
Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

287
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

8.8K

Área de la Ciencia:

  • Biología molecular
  • El metabolismo celular
  • La bioquímica

Sus antecedentes:

  • El objetivo de la rapamicina (TOR) es una serina / treonina quinasa crucial que regula el crecimiento celular y el metabolismo.
  • La señalización TOR es activada por nutrientes, factores de crecimiento y niveles de energía celular.
  • TOR existe en dos complejos distintos, TORC1 y TORC2, con diferentes funciones.

Objetivo del estudio:

  • Examinar e identificar todos los sustratos directos de TOR de mamíferos (mTOR).
  • Para aclarar cómo mTORC1 y mTORC2 fosforilan sustratos distintos.
  • Comprender el mecanismo de reclutamiento de sustratos por los complejos mTOR basado en datos estructurales.

Principales métodos:

  • Revisión exhaustiva de la literatura sobre la señalización mTOR.
  • Identificación de los sustratos mTOR directos.
  • Análisis de la información estructural relativa a los complejos mTOR.

Principales resultados:

  • Se han identificado numerosos sustratos directos para mTOR.
  • Se ha demostrado que mTORC1 y mTORC2 fosforilan conjuntos distintos de sustratos.
  • Se demostró que ambos complejos utilizan un motivo de fosforilación común.

Conclusiones:

  • mTORC1 y mTORC2, a pesar de una subunidad catalítica compartida, exhiben especificidad de sustrato.
  • Los dos complejos reclutan diferentes sustratos a través de mecanismos distintos.
  • Un motivo mínimo común es fosforilado por ambos complejos mTOR, lo que indica una regulación coordinada.