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Reacción en cadena microfluídica de eventos de flujo capilar estructuralmente programados
Mohamed Yafia1,2, Oriol Ymbern1,2, Ayokunle O Olanrewaju1,2,3
1Biomedical Engineering Department, McGill University, Montreal, Quebec, Canada.
Nature
|May 18, 2022
Resumen
Desarrollamos una reacción en cadena microfluídica (MCR) para el manejo autónomo y programable de líquidos en un chip. Esta innovación permite análisis y diagnósticos complejos sin equipos externos, allanando el camino para aplicaciones versátiles de laboratorio en chip.
Área de la Ciencia:
- Biotecnología
- Microfluidos
- Ingeniería Química
Sus antecedentes:
- Las reacciones en cadena son fundamentales para los procesos químicos y biológicos, pero las aplicaciones macroscópicas son limitadas.
- Los sistemas microfluídicos de laboratorio en un chip a menudo dependen de periféricos externos para la automatización.
- Los microfluidos capilares existentes carecen de una programabilidad avanzada para el manejo complejo de líquidos.
Objetivo del estudio:
- Introducir la reacción en cadena microfluídica (RCM) para el control de flujo capilar autónomo y programable.
- Para demostrar la capacidad de MCR para complejos algoritmos de manejo de líquidos en un solo chip.
- Para mostrar el potencial de MCR para dispositivos de laboratorio en un chip no atados y programados in situ.
Principales métodos:
- Impresión en 3D de chips monolíticos que integran las MCR.
- Utilizando la energía libre generada por la bomba de papel para el funcionamiento autónomo.
- Desarrollo de MCR para la propagación condicional y estructuralmente programada de eventos de flujo capilar.
Principales resultados:
- Liberación automática secuencial de 300 aliquotas a través de chips interconectados.
- Implementación exitosa de un protocolo de detección de anticuerpos contra el SARS-CoV-2.
- Se ha demostrado un ensayo de generación de trombina con submuestreo continuo y operaciones paralelas.
Conclusiones:
- La tecnología MCR ofrece un manejo autónomo de líquidos sin ataduras ni trabas.
- Los MCR codifican programas estructuralmente in situ, lo que permite dispositivos frugal y versátil de laboratorio en un chip.
- Este enfoque tiene amplias aplicaciones en el manejo de líquidos y el diagnóstico en el punto de atención.
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