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La deubiquitinasa USP8 tiene como objetivo el ESCRT-III para promover la división celular incompleta

Juliette Mathieu1, Pascale Michel-Hissier1, Virginie Boucherit1

  • 1Center for Interdisciplinary Research in Biology, Collège de France, PSL Research University, CNRS, Inserm, Paris, France.

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|May 19, 2022
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Resumen

La pérdida del gen USP8 en Drosophila hace que las divisiones celulares germinales se completen, mientras que su sobreexpresión conduce a divisiones incompletas. La ubiquitinación de las proteínas ESCRT-III por USP8 regula este interruptor de división celular.

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Área de la Ciencia:

  • Biología celular
  • Biología del desarrollo
  • La genética

Sus antecedentes:

  • Los gametos en muchos organismos se desarrollan en quistes germinales formados por divisiones celulares incompletas.
  • Comprender la regulación de la integridad de la división celular es crucial para la biología reproductiva.

Objetivo del estudio:

  • Investigar el papel de la deubiquitinasa USP8 en la regulación de la integridad de la división celular de la línea germinal en Drosophila.
  • Identificar los mecanismos moleculares mediante los cuales USP8 controla la citocinesis.

Principales métodos:

  • Utilizó Drosophila melanogaster como organismo modelo.
  • Manipulación genética empleada (nocaut genético y sobreexpresión) de USP8.
  • Investigó la localización y la función de las proteínas ESCRT-III (CHMP2B, Shrub/CHMP4).

Principales resultados:

  • La pérdida de USP8 transforma las divisiones germinales incompletas en completas.
  • La sobreexpresión USP8 invierte esto, promoviendo divisiones incompletas.
  • USP8 desubiquitina las proteínas ESCRT-III, afectando su reclutamiento en los puentes intercelulares.
  • El reclutamiento aberrante de ESCRT en los mutantes Usp8 conduce a la ruptura del quiste.

Conclusiones:

  • La ubiquitinación de ESCRT-III por USP8 actúa como un regulador clave que cambia entre las divisiones celulares completas e incompletas.
  • Este mecanismo es vital para la correcta formación e integridad del quiste de la línea germinal.