Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

DNA Microarrays02:34

DNA Microarrays

18.3K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
18.3K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.2K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.2K
Real Time RT-PCR02:57

Real Time RT-PCR

58.8K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
58.8K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Highly efficient and low-mosaicism <i>piggyBac</i> transgenesis platform for rapid founder phenotyping.

iScience·2026
Same author

Arterial calcification due to CD73 deficiency presenting with critical limb-threatening ischemia successfully treated with iliac endarterectomy and distal bypass.

Journal of vascular surgery cases and innovative techniques·2026
Same author

Midterm Outcomes of the Endurant Limb Fenestration With Internal Iliac Artery Repair and Enhanced Preservation (ELFIRE) Procedure.

Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists·2026
Same author

Tolerance to extracellular acidic pH facilitates tumor plasticity.

Cell reports·2026
Same author

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation.

Nature communications·2026
Same author

Transcriptome-based maturation assessment revealed pro-maturation transcription factors of cardiomyocytes.

Frontiers in bioengineering and biotechnology·2026

Video Experimental Relacionado

Updated: Sep 3, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.9K

Registradores moleculares para el seguimiento de eventos celulares

Nanami Masuyama1,2, Naoki Konno3, Nozomu Yachie1,4

  • 1School of Biomedical Engineering, Faculty of Applied Science and Faculty of Medicine, The University of British Columbia, Vancouver, BC, Canada.

Science (New York, N.Y.)
|July 28, 2022
PubMed
Resumen

Las cintas de ADN ofrecen un método novedoso para registrar eventos moleculares y celulares dinámicos dentro de los animales. Este enfoque innovador permite el seguimiento en tiempo real de los procesos biológicos para mejorar la investigación.

Más Videos Relacionados

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.2K
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

7.6K

Videos de Experimentos Relacionados

Last Updated: Sep 3, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.9K
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.2K
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

7.6K

Área de la Ciencia:

  • Biología molecular
  • La genética
  • Ciencias de los animales

Sus antecedentes:

  • Comprender los eventos dinámicos moleculares y celulares es crucial para la investigación biológica.
  • Los métodos actuales para el seguimiento de estos eventos in vivo pueden ser limitados.

Objetivo del estudio:

  • Explorar el potencial de las cintas de ADN para registrar eventos dinámicos moleculares y celulares en animales.

Principales métodos:

  • Desarrollo de la tecnología de cinta de ADN para el registro biológico.
  • Aplicación de cintas de ADN en modelos animales para capturar eventos celulares.

Principales resultados:

  • Se ha demostrado la viabilidad de utilizar cintas de ADN para registrar información biológica.
  • Captura exitosa de las actividades dinámicas moleculares y celulares.

Conclusiones:

  • Las cintas de ADN representan una nueva herramienta prometedora para el registro de eventos biológicos in vivo.
  • Esta tecnología podría revolucionar el estudio de la fisiología animal y las enfermedades.