Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

8.5K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
8.5K
ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

5.1K
The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
5.1K
Primary Active Transport01:29

Primary Active Transport

10.8K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would...
10.8K
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

3.9K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
3.9K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

4.8K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.8K
Energy to Drive Translocation01:37

Energy to Drive Translocation

2.1K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Inverted metal-free active template synthesis of rotaxanes via axle‑mediated macrocyclization.

Nature chemistry·2026
Same author

Trefoil polymers from a knotted synthon.

Nature chemistry·2026
Same author

Conformationally Switchable Molecular Trefoil Knot Assembled From 2,6-Bis(1,2,3-triazol-4-yl)pyridine (btp) Building Blocks.

Journal of the American Chemical Society·2026
Same author

Coordination Chemistry of a Star of David [2]Catenand.

Journal of the American Chemical Society·2026
Same author

Chiral catalysis-driven rotary molecular motors.

Nature chemistry·2026
Same author

Clean Electrospray Deposition of Porphyrin Molecules and Polyethylene Glycol Chains onto a Au(100) Surface.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same journal

On-Cell Detection of Polysaccharide One-Bond <sup>1</sup>J<sub>CH</sub> Couplings by Proton-Detected Solid-State NMR.

Journal of the American Chemical Society·2026
Same journal

Correction to "Unraveling the Effects of Fe Incorporation on High-Performance Water-Splitting Photoanodes".

Journal of the American Chemical Society·2026
Same journal

Proximity-Driven Protein Ligation Beyond the Concentration Limit.

Journal of the American Chemical Society·2026
Same journal

GraPhAI: Neural Networks for Solving Centrosymmetric Crystal Structures.

Journal of the American Chemical Society·2026
Same journal

Probing Stage Transition Kinetics in Li-Graphite Intercalation Compounds by Time-Resolved In Situ Solid-State NMR via <sup>13</sup>C Labeling.

Journal of the American Chemical Society·2026
Same journal

Dynamic Covalent Programming at DNA Base-Pairing Interfaces.

Journal of the American Chemical Society·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Sep 1, 2025

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
13:40

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

Published on: February 17, 2014

12.0K

Las bombas moleculares impulsadas por transamidación

Lorna Binks1, Chong Tian1, Stephen D P Fielden1

  • 1Department of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.

Journal of the American Chemical Society
|August 18, 2022
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores desarrollaron nuevas bombas moleculares sintéticas utilizando un mecanismo de trinquete de información gradual. Estas bombas secuestran de manera eficiente los sustratos macrocíclicos, lo que permite el ensamblaje secuencial controlado de rotaxanos complejos sin deshidratación intermedia.

Más Videos Relacionados

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
10:43

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Published on: December 3, 2010

22.3K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K

Videos de Experimentos Relacionados

Last Updated: Sep 1, 2025

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
13:40

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

Published on: February 17, 2014

12.0K
Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
10:43

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Published on: December 3, 2010

22.3K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K

Área de la Ciencia:

  • Química supramolecular
  • Química orgánica sintética
  • Nanotecnología

Sus antecedentes:

  • Las bombas moleculares son esenciales para la manipulación controlada de las moléculas.
  • Los métodos anteriores a menudo sufren de secuestro ineficiente o falta de control sobre el ensamblaje secuencial.
  • Los sustratos macrocíclicos requieren mecanismos específicos para la unión y liberación controladas.

Objetivo del estudio:

  • Para reportar una nueva clase de bombas moleculares sintéticas.
  • Demostrar un mecanismo de trinquete de información por etapas para el cerramiento cinético.
  • Para lograr un secuestro eficiente de sustratos macrocíclicos y una síntesis controlada de rotaxano.

Principales métodos:

  • Utilizó reacciones de plantilla activa entre la amina terminal de la bomba y el electrofilo de acilo.
  • Se utiliza la carboxilación y la conversión de carbamato en éster fenólico para ciclos de bombeo secuenciales.
  • Caracterización estructural de rotaxanos sintetizados mediante difracción de rayos X de un solo cristal.

Principales resultados:

  • Se han desarrollado bombas moleculares capaces de enganchar macrociclos a un hilo desde uno o ambos extremos.
  • Se obtiene una adición de anillo adicional por ciclo de bombeo por grupo acilo terminal.
  • Sintetizó un [4] rotaxano con tres macrociclos diferentes y un [5] rotaxano, confirmado por difracción de rayos X que muestra interacciones estabilizadoras.

Conclusiones:

  • Las nuevas bombas moleculares utilizan efectivamente un mecanismo de trinquete de información gradual.
  • La ausencia de estados de pseudorotaxano asegura una carga secuencial eficiente y controlada de los macrociclos.
  • Esta metodología facilita la síntesis precisa de rotaxanos complejos definidos por secuencia.