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Percepción de las auxinas ABP1-TMK para la fosforilación global y la canalización de las auxinas

Jiří Friml¹, Michelle Gallei², Zuzana Gelová²

¹Institute of Science and Technology Austria (ISTA), Klosterneuburg, Austria. jiri.friml@ista.ac.at.

|September 7, 2022

Resumen

La fitohormona de la auxina

Área de la Ciencia:

Biología vegetal
Señales moleculares
Biología celular

Sus antecedentes:

Se entiende la reprogramación nuclear de la auxina, pero los mecanismos de señalización rápida siguen sin estar claros.
Se debate el papel de la proteína 1 de unión a la auxina (ABP1) como receptor de la auxina.
Los efectos de las auxinas rápidas incluyen la regulación del flujo iónico y la fosforilación de proteínas.

Objetivo del estudio:

Investigar el papel de ABP1 en la señalización rápida de la auxina.
Para identificar el mecanismo del receptor de auxina para las respuestas celulares ultrarrápidas.
Aclarar la función de ABP1 en el transporte de auxinas y el desarrollo de las plantas.

Principales métodos:

Se ha investigado la unión de ABP1 secretado a la auxina a pH ácido.
Se utilizaron mutantes de Arabidopsis thaliana (abp1, tmk1) para evaluar la función genética.
Se utilizó una variante ABP1 con deficiencia de unión a las auxinas (ABP1 ((M2X)) para estudios de complementación.

Principales resultados:

El ABP1 secretado se une a la auxina en el apoplasto ácido.
La ABP1 y la TMK1 localizada en la membrana plasmática son esenciales para las respuestas fosfóricas ultrarrápidas inducidas por auxinas.
Los mutantes abp1 y tmk1 presentan defectos en el transporte de auxinas, la formación de vasculatura y la regeneración.
La capacidad de unión a las auxinas de ABP1 es fundamental para complementar estos defectos de desarrollo.

Conclusiones:

ABP1 funciona como el receptor de auxina para la señalización de la superficie celular mediada por TMK1.
Esta vía de señalización regula las respuestas globales de fosfo y la canalización de auxinas.
ABP1 es crucial para las respuestas rápidas de las auxinas y el desarrollo de las plantas, resolviendo preguntas de larga data sobre su función de receptor.

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