Controles de señalización TOR-FIE-PRC2 basados en glucosa para el desarrollo de la planta

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Ruiqiang Ye^1,2, Meiyue Wang^3,4,5, Hao Du^6,7

¹Department of Molecular Biology and Centre for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, USA. ye@molbio.mgh.harvard.edu.

|September 14, 2022

Resumen

El objetivo de la cinasa de la rapamicina (TOR) activada por la glucosa en las plantas controla el destino y el desarrollo celular mediante la regulación de la metilación de las histonas. Esta vía de señalización de nutrientes afecta la organogénesis de las plantas y la transición a la floración.

Área de la Ciencia:

Biología vegetal
La epigenética
La señalización molecular

Sus antecedentes:

Los nutrientes y la energía son cruciales para el desarrollo de las plantas.
El objetivo de la cinasa de rapamicina (TOR) integra las señales de nutrientes para controlar el crecimiento.
El papel de TOR en las transiciones y la diferenciación del desarrollo no se entiende completamente.

Objetivo del estudio:

Investigar cómo la TOR quinasa activada por glucosa influye en las modificaciones epigenéticas y los programas de desarrollo en Arabidopsis thaliana.
Identificar y caracterizar los objetivos TOR involucrados en la regulación del destino y el desarrollo celular.

Principales métodos:

Análisis genómico de la trimetilación de la histona H3 en K27 (H3K27me3).
Identificación del endosperma independiente de la fertilización (FIE) como objetivo TOR.
Análisis de mutación en el sitio de fosforilación de FIE.
Análisis de la reprogramación del transcriptoma.
Investigación de la vía de señalización del complejo represivo de glucosa-TOR-FIE-Polycomb 2 (PRC2).

Principales resultados:

La TOR quinasa activada por glucosa controla los niveles de H3K27me3 en todo el genoma en Arabidopsis.
El endosperma independiente de la fertilización (FIE) es un objetivo directo de TOR, y su fosforilación por TOR impulsa la translocación nuclear.
La interrupción de la fosforilación de FIE anula H3K27me3, altera el transcriptoma y perjudica la organogénesis.
La vía glucosa-TOR-FIE-PRC2 regula la transición floral inducida por la vernalización.

Conclusiones:

El eje de señalización glucosa-TOR-FIE-PRC2 actúa como punto de control nutricional.
Esta vía silencia epigenéticamente los genes que controlan el destino de las células madre y el patrón de los órganos.
La señalización de nutrientes reprograma directamente el epigenoma, influyendo en las transiciones de desarrollo en las plantas.

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