Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
CRISPR01:59

CRISPR

52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

165
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
165
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

104
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
104
Homologous Recombination02:31

Homologous Recombination

50.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.9K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Metal-Phenolic Coatings Enable Universal Design of Spherical Nucleic Acids.

Angewandte Chemie (International ed. in English)·2026
Same author

Correction to "DNA-Mediated Cellular Delivery of Functional Enzymes".

Journal of the American Chemical Society·2026
Same author

High-χ Block Copolymer Nanoreactors for the Confined Synthesis of Size-Controlled Nanoclusters.

ACS nano·2026
Same author

Programmable Stepwise Heteroepitaxial Growth of Colloidal Crystals With Different Phases.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Simplex-based model for nanoparticle grain identification in four-dimensional scanning transmission electron microscopy data.

Journal of microscopy·2026
Same author

High-entropy 1D halide perovskite piezoelectrics found by megalibrary synthesis and rapid nonlinear optical screening.

Science advances·2026
Same journal

A Lithium Superionic Conductor Softened by Nonmetal-Chlorine Chemical Bonds.

Journal of the American Chemical Society·2026
Same journal

A Ferrocene Metal-Ligand Triplet Diradical with a Terminal Iminyl Group Discovered by Time-Resolved Mid-Infrared Spectroscopy.

Journal of the American Chemical Society·2026
Same journal

Regulating Li-Ion Transport via Solvent and Ion Clustering Using Ternary Salts in Nonfluorinated Solvents for Extended Cyclability of Zero-Excess Lithium-Metal Batteries.

Journal of the American Chemical Society·2026
Same journal

Terahertz-Field-Induced Dissociation of Frenkel Excitons in Organic Semiconductors.

Journal of the American Chemical Society·2026
Same journal

Interplay between Slow Chirality Inversion and Slow Guest Uptake in a Triple-Helical Closed-Cage Metallocryptand.

Journal of the American Chemical Society·2026
Same journal

Controlled Sulfane Sulfur Delivery via Allyl Disulfide Rearrangement-Mediated Thiosulfoxide Formation.

Journal of the American Chemical Society·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Aug 26, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.7K

Ácidos nucleicos esféricos CRISPR

Chi Huang1, Zhenyu Han1, Michael Evangelopoulos2

  • 1Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, Illinois 60208-3113, United States.

Journal of the American Chemical Society
|October 6, 2022
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores desarrollaron nuevos ácidos nucleicos esféricos CRISPR (SNA) para una edición eficiente del genoma. Estos Cas9 ProSNA mejoran la entrega celular y la eficiencia de edición sin métodos duros, ampliando las aplicaciones de CRISPR.

Más Videos Relacionados

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.3K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.5K

Videos de Experimentos Relacionados

Last Updated: Aug 26, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

12.7K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.3K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.5K

Área de la Ciencia:

  • Biotecnología
  • Biología molecular
  • Nanotecnología

Sus antecedentes:

  • La eficiencia de la edición del genoma CRISPR/Cas9 se ve obstaculizada por los desafíos en la entrega de componentes en células y tejidos.
  • Los ácidos nucleicos esféricos (SNA) ofrecen potencial para una mayor absorción celular, pero no se han aplicado a la edición de genes.

Objetivo del estudio:

  • Diseñar y evaluar una nueva clase de SNA CRISPR para mejorar la edición del genoma.
  • Para mejorar la entrega celular y la localización nuclear de los componentes de CRISPR-Cas9.

Principales métodos:

  • Cas9 ProSNAs sintetizados: núcleos de proteínas Cas9 densamente modificados con exteriores de ADN, precargados con ARN guía.
  • Los péptidos GALA incorporados y las señales de localización nuclear mejoran el escape endosómico y la orientación nuclear.
  • Se evaluó la estabilidad frente a la digestión de la proteasa y se evaluó la eficiencia de la edición del genoma en múltiples líneas celulares.

Principales resultados:

  • Cas9 ProSNAs demostró una mayor absorción celular sin electroporación o agentes de transfección.
  • Las construcciones mostraron estabilidad frente a la degradación de la proteasa.
  • Logró eficiencias de edición del genoma que van desde el 32% hasta el 47% en diferentes líneas celulares.

Conclusiones:

  • Los nuevos SNAs CRISPR (Cas9 ProSNAs) superan las principales barreras de entrega para la edición del genoma.
  • Estas nanoestructuras mejoran significativamente la absorción celular y la eficiencia de edición.
  • Este avance tiene el potencial de ampliar las aplicaciones y el impacto de la tecnología CRISPR-Cas9.