Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.4K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.7K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.4K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.4K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

3.8K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.8K
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

8.7K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.7K
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

12.3K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.3K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Titanium dioxide nanoparticles relieve biochemical dysfunctions of fifth-instar larvae of silkworms following exposure to phoxim insecticide.

Chemosphere·2012
Same author

Mechanisms of prostate atrophy after LHRH antagonist cetrorelix injection: an experimental study in a rat model of benign prostatic hyperplasia.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban·2012
Same author

Simulation and experimental investigation of structural dynamic frequency characteristics control.

Sensors (Basel, Switzerland)·2012
Same author

Chronic clomipramine treatment restores hippocampal expression of glial cell line-derived neurotrophic factor in a rat model of depression.

Journal of affective disorders·2012
Same author

Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2012
Same author

Identification of Sare0718 as an alanine-activating adenylation domain in marine actinomycete Salinispora arenicola CNS-205.

PloS one·2012

Video Experimental Relacionado

Updated: Aug 23, 2025

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

19.9K

La coalescencia multifásica media la activación de la vía Hippo

Li Wang1, Kyungsuk Choi1, Ting Su1

  • 1Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Cell
|November 1, 2022
PubMed
Resumen

Los científicos han descubierto una nueva forma de controlar los condensados biomoleculares sin disolverlos. Esto implica reguladores aguas arriba de la vía de señalización de Hippo formando condensados distintos que se fusionan para regular las funciones celulares.

Palabras clave:
La AMOTEl camino del hipopótamoKIBRA (en inglés)Se trata de la siguiente:YAP (en inglés)Condensados biomolecularescontacto entre célulasEl citosqueletode varias fasesestrés osmótico

Más Videos Relacionados

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.0K
Culturing and Manipulation of O9-1 Neural Crest Cells
08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

10.2K

Videos de Experimentos Relacionados

Last Updated: Aug 23, 2025

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

19.9K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.0K
Culturing and Manipulation of O9-1 Neural Crest Cells
08:32

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

10.2K

Área de la Ciencia:

  • Biología celular
  • La bioquímica
  • Biología molecular
  • Transducción de señales

Sus antecedentes:

  • La función del condensado biomolecular a menudo está limitada por la disolución.
  • Los mecanismos para suprimir la actividad del condensado sin disolución siguen siendo en gran medida desconocidos.
  • La vía de señalización del hipopótamo juega un papel crucial en el crecimiento celular y el tamaño del órgano.

Objetivo del estudio:

  • Investigar si la función del condensado biomolecular puede suprimirse sin disolución.
  • Para aclarar el papel de los reguladores aguas arriba en la modulación de la actividad de la vía de señalización Hippo.
  • Comprender los principios de la organización multifásica en los sistemas biológicos.

Principales métodos:

  • Investigó la formación e interacción de condensados formados por SLMAP, AMOT y KIBRA.
  • Se utilizaron técnicas para observar la coalescencia del condensado y su efecto en la función del complejo STRIPAK.
  • Analizó la regulación aguas arriba de la vía de señalización Hippo bajo diferentes condiciones celulares.

Principales resultados:

  • Los reguladores aguas arriba de la vía Hippo forman condensados funcionalmente antagónicos.
  • SLMAP forma condensados hipo-inactivantes, mientras que AMOT y KIBRA forman condensados hipo-activantes.
  • La coalescencia de estos condensados opuestos en una fase común inhibe la función del complejo STRIPAK, suprimiendo la actividad de la vía sin disolución.

Conclusiones:

  • Se presenta un nuevo paradigma para regular la actividad de condensado biomolecular sin disolución.
  • La organización multifásica que involucra condensados funcionalmente antagónicos proporciona un mecanismo para ajustar los procesos celulares.
  • Este estudio ofrece información sobre la regulación aguas arriba de la vía de señalización del hipopótamo y el comportamiento del condensado.