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Un mecanismo para el cierre del ARN del SARS-CoV-2 y su inhibición por inhibidores análogos de nucleótidos

Liming Yan¹, Yucen Huang², Ji Ge³

¹MOE Key Laboratory of Protein Science, School of Medicine, Tsinghua University, Beijing, China.

|November 6, 2022

Resumen

La polimerasa del SARS-CoV-2

Área de la Ciencia:

Virología y Biología Molecular
Biología estructural
Descubrimiento de drogas

Sus antecedentes:

El cierre del ARN viral es crucial para la replicación del SARS-CoV-2.
Comprender el mecanismo de cierre del ARN es clave para desarrollar estrategias antivirales.

Objetivo del estudio:

Para dilucidar el mecanismo de cierre del ARN del SARS-CoV-2 a resolución atómica.
Identificar posibles objetivos farmacológicos y diseñar estrategias para nuevos agentes antivirales.

Principales métodos:

Análisis estructural detallado del dominio NiRAN en la polimerasa del SARS-CoV-2.
Investigación de la interacción entre los inhibidores analógicos de nucleótidos y las proteínas virales.

Principales resultados:

El dominio NiRAN cataliza la RNAilación de nsp9 y la posterior formación de la capa (GpppA).
Los inhibidores analógicos de nucleótidos se unen a nsp9 dentro de un nuevo "Nuc-pocket" en NiRAN, inhibiendo el cierre del ARN.
El bucle S en NiRAN exhibe cambios conformacionales, lo que sugiere un mecanismo de inhibición de "inducción y bloqueo".

Conclusiones:

El estudio revela los detalles atómicos del cierre del ARN del SARS-CoV-2.
Los hallazgos proporcionan información sobre el mecanismo de acción de los inhibidores analógicos de nucleótidos.
Se propone una nueva estrategia para diseñar medicamentos antivirales contra el SARS-CoV-2 dirigidos a la limitación del ARN.

Palabras clave:

En el caso del SARS-CoV-2 el límite máximo Crio-EM Inhibidor de los análogos de nucleótidos

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