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Un transportador microbiano del antioxidante ergotioneína en la dieta

Daniel G Dumitrescu¹, Elizabeth M Gordon², Yekaterina Kovalyova¹

¹Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA; Department of Chemistry, Yale University, New Haven, CT 06520, USA; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA.

|November 8, 2022

Resumen

Helicobacter pylori elimina el antioxidante ergotioneína (EGT) del huésped mediante el transportador EgtUV. Este EGT dietético ayuda a la colonización microbiana e impacta la salud intestinal, revelando un nuevo mecanismo de regulación redox.

Área de la Ciencia:

Microbiología
La bioquímica
Salud humana

Sus antecedentes:

Los tiolos de bajo peso molecular (LMW) son antioxidantes cruciales para el equilibrio redox celular.
Muchos microbios, incluido Helicobacter pylori, carecen de vías para sintetizar estos tiolos esenciales.
La ergotioneína dietética (EGT) es abundante en los tejidos humanos y está relacionada con la mitigación del riesgo de enfermedad.

Objetivo del estudio:

Para investigar cómo Helicobacter pylori mantiene la homeostasis redox a pesar de la falta de biosíntesis de LMW-tiol.
Identificar la fuente y el mecanismo de adquisición de ergotioneína por H. pylori.
Explorar las implicaciones más amplias del metabolismo microbiano de la ergotioneína en el intestino humano.

Principales métodos:

Metabolomía guiada por la reactividad para identificar los tiolos LMW en H. pylori.
Caracterización genética y bioquímica del transportador EgtUV.
Ensayos de colonización in vivo y análisis de la microbiota fecal humana.

Principales resultados:

La ergotioneína (EGT) fue identificada como un tiol LMW inusual utilizado por H. pylori.
H. pylori adquiere EGT del entorno huésped a través del transportador de casete selectivo de unión de ATP EgtUV.
EgtUV confiere una ventaja competitiva para la colonización de H. pylori y se conserva en los microbios intestinales.
Las bacterias intestinales humanas metabolizan el EGT, contribuyendo potencialmente a la producción de N-óxido de trimetilamina.

Conclusiones:

H. pylori emplea una estrategia antioxidante derivada del huésped, adquiriendo EGT a través del transportador EgtUV.
La competencia interreino por el EGT alimentario representa un factor importante en la ecología microbiana intestinal y en la salud humana.
Este estudio descubre un nuevo mecanismo de regulación redox microbiana que involucra interacciones huésped-micróbio.

Palabras clave:

El transportador ABC Helicobacter pylori y sus derivados Ergothioneine, también conocido como ergothioneine microbios huésped Tiol de bajo peso molecular el metabolismo el microbioma El estrés oxidativo Regulación de oxidación y oxidación

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