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Base molecular de los conjuntos supramoleculares anti-fago de RADAR

Yina Gao¹, Xiu Luo¹, Peipei Li²

¹CAS Key Laboratory of Infection and Immunity, National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

|February 10, 2023

Resumen

La defensa RADAR bacteriana utiliza las proteínas RdrA y RdrB para editar el ARN. Las estructuras Cryo-EM revelan cómo estas proteínas se ensamblan para translocar y modificar el ARN, proporcionando información sobre los mecanismos antifágicos.

Área de la Ciencia:

Biología molecular
Biología estructural
La bioquímica

Sus antecedentes:

La edición de ARN de adenosina a inosina está implicada en la defensa antifago bacteriana.
El sistema RADAR comprende la adenosina trifosfatasa (RdrA) y la adenosina desaminasa (RdrB).

Objetivo del estudio:

Para aclarar la base estructural del sistema RADAR.
Comprender el mecanismo de la translocación y desaminación del ARN en la defensa bacteriana contra los fagos.

Principales métodos:

Se utilizó la crio-microscopía electrónica (cryo-EM) para determinar las estructuras de RdrA, RdrB y sus complejos.
Se realizaron ensayos bioquímicos para evaluar los aspectos funcionales del sistema.

Principales resultados:

RdrB forma una jaula dodecamérica, mientras que RdrA existe como anillos tetradecaméricos (autoinhibidos) y heptaméricos (activos).
Los datos estructurales sugieren que la translocación de ARN a través de RdrA está acoplada a la unión de ATP.
Múltiples anillos RdrA se acoplan a la jaula RdrB, alineando los sitios catalíticos para la translocación y desaminación acopladas de ARN.

Conclusiones:

El estudio revela un nuevo mecanismo de acoplamiento enzimático para la edición de ARN y la defensa antifágico.
El ensamblaje supramolecular de RdrA y RdrB es crucial para la función del sistema RADAR.

Palabras clave:

La ATPasa Desaminasa de la adenosina De la adenosina a la inosina Defensa antifágico El RADAR Edición de ARN Ensamblaje supramolecular

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