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Integración completa de la química de transferencia de carbenos en la biosíntesis

Jing Huang^1,2,3, Andrew Quest^4,5, Pablo Cruz-Morales^1,2,6

¹Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

|May 3, 2023

Resumen

Este estudio desarrolló una plataforma microbiana para la biosíntesis, permitiendo reacciones de transferencia de carbenos intracelulares. Este enfoque permite la producción rentable de nuevas moléculas orgánicas utilizando el metabolismo celular.

Área de la Ciencia:

Biotecnología
Biología sintética
Química orgánica

Sus antecedentes:

La biosíntesis ofrece una vía sostenible hacia productos naturales y nuevos.
Los sistemas biológicos carecen de muchas reacciones sintéticas, lo que limita el alcance del producto.
La transferencia anterior de carbenos en la célula requería reactivos exógenos, lo que dificultaba la ampliación.

Objetivo del estudio:

Desarrollar una plataforma microbiana para las reacciones de transferencia de carbenos abiológicos intracelulares.
Para permitir la biosíntesis rentable de moléculas orgánicas complejas.
Para ampliar la gama de productos accesibles a través del metabolismo celular.

Principales métodos:

Diseñado Streptomyces albus para producir el diazoester azaserina intracelularmente.
Se utiliza la azaserina intracelular como donante de carbenos para la ciclopropanación del estireno.
Utilizó mutantes P450 diseñados con cofactores nativos para la catálisis.

Principales resultados:

Se produjo con éxito azaserina a través de un grupo de genes biosintéticos microbianos.
Se ha demostrado la transferencia intracelular de carbenos para la ciclopropanación de estireno.
Se logró una buena diastereoselectividad y un rendimiento moderado utilizando P450 modificados.

Conclusiones:

Se estableció una plataforma microbiana escalable para las reacciones de transferencia de carbenos intracelulares.
Amplió el alcance de los productos fabricables por el metabolismo celular.
Facilitó la funcionalidad de los productos naturales y nuevos para la naturaleza.

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