Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

The Two-State Receptor Model01:29

The Two-State Receptor Model

2.0K
The two-state receptor model explains a drug's interaction with receptors, such as G protein-coupled receptors and ligand-gated ion channels, to induce or inhibit a biological response. When no natural ligands are present, a receptor exists in an equilibrium of inactive (Ri) and active (Ra) conformations. The inactive form does not produce a response, while the active form generates a basal effect known as constitutive activity.
The binding affinity of a drug determines its interaction with...
2.0K
Protein Folding01:22

Protein Folding

118.6K
Overview
118.6K
Conserved Binding Sites01:49

Conserved Binding Sites

4.3K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.3K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

7.9K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
7.9K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.5K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.5K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

RNA G-quadruplexes function as a tunable switch of FUS phase separation.

Nucleic acids research·2026
Same author

Dissecting hydrogen bond energetics to answer the age-old question: "How much do hydrogen bonds contribute to enzymatic catalysis?"

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Unraveling the Stereochemical Complexity of Phosphorothioate-Modified Oligonucleotides Using Analytical Technologies.

Nucleic acid therapeutics·2026
Same author

Assessing the contribution of rare DNA states to cancer mutational signatures using sequence-specific conformational fingerprinting.

Nature communications·2026
Same author

High-throughput biochemical phenotyping of SHP2 variants reveals the molecular basis of diseases and allosteric drug inhibition.

bioRxiv : the preprint server for biology·2026
Same author

Thermodynamic prediction of RNA cellular activity from sequence via conformational ensembles.

Cell·2026

Video Experimental Relacionado

Updated: Jul 30, 2025

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
10:59

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Published on: August 17, 2022

3.3K

Las propensiones conformacionales del ARN determinan la actividad celular

Megan L Ken1, Rohit Roy2, Ainan Geng1

  • 1Department of Biochemistry, Duke University School of Medicine, Durham, NC, USA.

Nature
|May 17, 2023
PubMed
Resumen
Este resumen es generado por máquina.

La actividad celular se basa en las interacciones biomoleculares, que están influenciadas por cambios conformacionales. Este estudio cuantifica estos cambios en el ARN TAR del VIH-1, relacionándolos con la afinidad de unión y la transactivación viral.

Más Videos Relacionados

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.0K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.0K

Videos de Experimentos Relacionados

Last Updated: Jul 30, 2025

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
10:59

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Published on: August 17, 2022

3.3K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.0K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.0K

Área de la Ciencia:

  • Biología molecular
  • La biofísica
  • Biología estructural

Sus antecedentes:

  • Los procesos celulares dependen de las interacciones biomoleculares que forman complejos activos.
  • Los contactos intermoleculares median estas interacciones, y su interrupción altera la fisiología celular.
  • Las interacciones biomoleculares a menudo requieren cambios conformacionales, que afectan la afinidad de unión y la actividad celular.

Objetivo del estudio:

  • Alterar y cuantificar sistemáticamente las propensiones conformacionales del ARN TAR del VIH-1.
  • Establecer el papel de las propensiones conformacionales basadas en el conjunto en la actividad celular.
  • Investigar cómo los estados conformacionales influyen en la afinidad de unión y la transactivación dependiente del Tat del VIH-1.

Principales métodos:

  • Alteración sistemática de las conformaciones del ARN TAR del VIH-1.
  • Medición cuantitativa de las propensiones conformacionales.
  • Evaluación de las afinidades de unión a la región de unión al ARN de la proteína Tat del VIH-1.
  • Medición de la transactivación dependiente del Tat del VIH-1 en modelos celulares.

Principales resultados:

  • Las propensiones conformacionales del ARN TAR del VIH-1 fueron alteradas y determinadas sistemáticamente.
  • Estas propensiones predijeron con precisión las afinidades de unión entre el ARN TAR y la proteína Tat.
  • El estudio predijo con éxito la extensión de la transactivación dependiente del Tat del VIH-1.
  • Se identificó un estado de conformación de ARN excepcionalmente raro y de corta duración como motor de un proceso celular.

Conclusiones:

  • Las propensiones conformacionales basadas en el conjunto juegan un papel crítico en la actividad celular.
  • La comprensión de la dinámica conformacional es esencial para modelar la energética de enlace biológico.
  • Este trabajo proporciona un marco cuantitativo para evaluar el impacto de los estados conformacionales en la función biológica.
  • Los hallazgos destacan la importancia de los estados conformacionales raros en los procesos biológicos.