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Wei Tsing, un canal de iones expresado por el pericíclo, protege la estela para conferir resistencia a la raíz del

Wei Wang¹, Li Qin², Wenjing Zhang³

¹State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572025, China.

|June 9, 2023

Resumen

Los investigadores descubrieron WeiTsing (WTS), un nuevo gen que confiere una resistencia de amplio espectro a la raíz en las plantas. Este gen codifica un canal iónico permeable al calcio crucial para activar las respuestas inmunes de las plantas contra la infección por Plasmodiophora brassicae (Pb).

Área de la Ciencia:

Patología vegetal
Biología molecular
La genética

Sus antecedentes:

Las raíces de las plantas son susceptibles a los microbios patógenos, lo que lleva a importantes pérdidas de cultivos.
La enfermedad de Clubroot, causada por Plasmodiophora brassicae (Pb), devasta los cultivos crucíferos en todo el mundo.

Objetivo del estudio:

Identificar y caracterizar un nuevo gen que confiere resistencia a la enfermedad de clubroot.
Aclarar el mecanismo molecular subyacente a la inmunidad de las plantas contra Plasmodiophora brassicae.

Principales métodos:

Aislamiento y caracterización del gen WeiTsing (WTS) de la Arabidopsis.
Análisis de la expresión de WTS en respuesta a la infección por Pb.
Generación de Brassica napus transgénica que expresa el gen WTS.
Microscopía cryoelectrónica (cryo-EM) para el análisis estructural.
Electrofisiología para determinar la actividad del canal.
Mutagénesis guiada por la estructura para evaluar el papel de la actividad del canal.

Principales resultados:

El gen WTS se activa por transcripción en el pericículo tras la infección por Pb, impidiendo la entrada del patógeno en la estela.
El Brassica napus transgénico con WTS exhibió una fuerte resistencia al Pb.
WTS codifica una pequeña proteína localizada en el retículo endoplasmático (ER) e induce respuestas inmunes.
Cryo-EM reveló una estructura pentamérica única con un poro central, que funciona como un canal catiónico permeable al calcio.
La actividad del canal de WTS es esencial para activar las defensas de la planta.

Conclusiones:

El WTS es un gen de resistencia de raíz en plantas de amplio espectro.
El WTS funciona como un canal iónico, análogo a los resistosomas, crucial para iniciar la señalización inmune en el pericíclo.
Los hallazgos proporcionan un nuevo objetivo para el desarrollo de cultivos resistentes a las enfermedades.

Palabras clave:

canal permeable al calcio la raíz del palo pericíclo Enfermedad de la raíz

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